RT-qPCR analysis is critical to success in knockdown experiments. Learn about how to rapidly troubleshoot your knockdown experiment from the RT-qPCR results.
Learn how antisense oligonucleotides (ASO) are maximizing treatment efficiency and reducing off-target effects in combinatorial therapies.
In this blog post, we share the highlights of our CRISPRmod CRISPR interference (CRISPRi) system, comparing the activity of Dharmacon’s novel CRISPRi construct dCas9-SALL1-SDS3 to dCas9-KRAB.
Getting started with CRISPR whole genome screening has never been easier with the NEW Dharmacon All-in-one CRISPR platform.
Design and order a plasmid DNA donor kit for insertion of a fluorescent marker
Arrayed siRNA screens are a powerful tool to look at very precise perturbations in cellular pathways.
Overview of HAP1 cells, their origins, and how we produce knockouts in these cell lines to see how they can assist in your research.
T-cell receptors (TCRs) are crucial components of T cell immunity, enabling the detection and subsequent elimination of abnormal, cancerous and infected cells. Dysfunctional T cell receptor signaling can lead to various immune deficiency or autoimmune diseases. In cancer treatment the study and modification of T cell receptor signaling is important for CAR-T cell therapy as well as modulation of the tumor microenvironment (TME). Modulating T cell activation can be important to avoid rejection of organ or stem cell transplants by the immune response.
Cancer is often characterized by dysregulation in cellular pathways and the checkpoints which control cell proliferation, growth, differentiation, and apoptosis. Within the genome there are two types of genes which carefully control cell proliferation and cell death, which are often the driving force behind oncogenesis.