Large-scale gain-of-function screens have not yet shown the same popularity as loss-of function experiments, mainly due to logistical complications of using the traditional reagents (ORFs). Here we explain the hurdles to setting up a high-throughput ORF screen, and how CRISPRa offers a new solution that can expand the screening toolbox to maximize your window of discovery.
A discussion of novel methodology and research in primary human monocytes recently published by the labs of Dr. Alex Marson and Dr. Nevan Krogan.
Need to incorporate human cell models in your work but unsure where to begin? The use of iPSCs as a model system is a smart place to start.
Detecting proteins with confidence starts with antibody validation. Here, we've outlined the key points.
Learn about Horizon's CRISPRsc (CRISPR single cell) platform that combines the flexibility of pooled screening with the power of transcriptomics at single cell resolution.
Gene modulation is now more flexible and accessible thanks to Horizon's new CRISPRmod CRISPRi portfolio. The system leverages an inactive Cas9 complex to block transcription resulting in precision targeted gene down regulation. Read more about our newest offering:
A review of two studies demonstrating that large-scale pooled base editor screens can separate pathogenic from benign mutations and identify variants that are associated with altered cellular growth or response to DNA-damaging agents.
We’re happy to discuss your project in English, French, German, Italian, or Spanish; and have teams in both the US and Europe to provide flexible service hours.
The promise of base editing showcases the ingenuity of scientists in their inexorable quest to precisely and safely engineer the genome.
Review of publications using HAP1 cells to set up validation assays beyond the typical western blot.