Learn about methods for CRISPR activation and determine the best path for your preferred cell model and experimental parameters.
If you've followed our guide to planning a successful genome editing experiment, then you'll hopefully be working in optimal conditions, and have a good idea of your guide's editing efficiency. This number should give you a rough idea of how many clones you're going to have to screen to find a targeted clones.
In this blog, we take a look at ClaretBio’s app note on using Horizon’s cfDNA reference standards in the development of their novel sample prep kit.
Learn about the MLR assay and why it is an essential component of the compound screening pipeline for drug development.
Describes methods of testing your cell culture for retroviral recombination.
CRISPR gene editing and siRNA gene silencing require the efficient delivery of the necessary reagents. This is easily achieved by optimizing delivery conditions. In this blog post we discuss techniques for optimization of a reverse transfection conditions and how to set up a 96 well plate to test 30 transfection conditions.
Learn how to examine your NGS data to investigate false positives and negatives, and potential downstream applications
Learn how to use Edit-R CRISPR reagents from Dharmacon to make functional knockout models in human iPSCs.