siRNA Reagents and Solutions
Silence your target gene across a wide range of cell types, with over 20 years of trusted Dharmacon™ siRNA design and synthesis
Gene knockdown with siRNA is a simple and reliable tool for functional gene silencing; applicable across a broad range of cells and experiments.
Dharmacon chemically modified and algorithmically designed siRNA enhance on-target performance and delivery. Choose from our predesigned siRNA or design your own with specific modifications against your desired target accession.
Find the right siRNA for your experiment
Predesigned siRNA reagents
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ON-TARGETplus™ 2.0 siRNA
Built of next-generation design to deliver exceptional knockdown with minimal off-targets and maximum isoform coverage. -
Accell™ 2.0 siRNA
Self-delivering siRNA with enhanced algorithmic design for transfection-free delivery in difficult-to-transfect models. -
ON-TARGETplus™ siRNA
Advanced chemically modified siRNA delivering high-specificity knockdown with reduced off-target activity. -
Accell™ siRNA
Self-delivering siRNA for gene silencing in difficult-to-transfect cells, no transfection needed. -
siGENOME™ siRNA
Trusted gene silencing backed by the longest-standing siRNA guarantee on the market. -
Lincode™ siRNA
Knockdown long, non-coding RNA genes with specialized siRNA, modified to ensure specificity.
Design and order customized siRNAs
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Custom siRNA
Easy ordering of standard, Dharmacon enhanced, and chemically modified siRNAs. -
Custom siRNA design center
Design custom siRNA for any gene or species with our siDESIGN Center. -
Custom siRNA SMARTpools
Custom siRNA SMARTpool reagents for targets other than our standard offerings like specific isoforms or alternative species.
Complete your siRNA experiment with controls, additional reagents and libraries
Why choose Dharmacon reagents siRNA?
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Trusted for over 20 years
a proven name in high-quality siRNA design and synthesis.
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Guaranteed gene silencing
ON-TARGETplus 2.0, ON-TARGETplus, Accell 2.0, Accell, and siGENOME siRNAs are guaranteed to silence target gene expression by at least 75% at the mRNA level.*
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Flexible formats
choose predesigned siRNA, customize siRNA against unique accessions, or add chemical modifications.
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Broad applicability
effective across a wide range of cell lines and experimental designs.
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Minimized off-target effects
SMARTselection design algorithm provides a high degree of confidence that the targeted gene will be the only gene silenced.
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Pooling for improved effectiveness
SMARTpool siRNA reagents combines four gene-specific siRNAs for enhanced silencing and reduced false negatives.
Reducing off-targets maximizes specificity
A successful siRNA knockdown experiment must be both effective and specific. Our SMARTselection design algorithm, which is used across ON-TARGETplus 2.0, Accell 2.0, ON-TARGETplus, Accell, siGENOME, and Lincode siRNA, provides a high degree of confidence that only the targeted gene will be silenced.
The primary source of siRNA-mediated off-targets is the seed region (nucleotides 2–7), which uses the microRNA pathway to induce non-specific gene silencing via interactions within the 3' UTR. SMARTselection incorporates seed region filters and seed frequency analysis to minimize microRNA-like off-target activity for improved specificity.
Which siRNA is right for you?
We offer multiple predesigned product lines across human, mouse, and rat genomes. Use the table below to determine the optimal siRNA for your needs.
| Feature / Attribute | ON-TARGETplus 2.0 siRNA | Accell 2.0 siRNA | ON-TARGETplus siRNA | siGENOME siRNA | Lincode siRNA |
|---|---|---|---|---|---|
| Key Benefit | Next-generation specificity with minimized off-target effects and improved consistency | Next-generation specificity to target silencing in difficult-to-transfect cells | High specificity for reduced off-targets + efficient silencing | Cost-effective, efficient silencing | Highly specific knockdown of long noncoding RNA (lncRNA) |
| Gene Coverage | Predesigned for Human, Mouse, and Rat protein-coding genes | Predesigned for Human, Mouse, and Rat protein-coding genes | Predesigned for Human, Mouse, and Rat protein-coding genes | Predesigned for Human, Mouse, and Rat protein-coding genes | Predesigned for Human and Mouse lncRNA |
| Best Use Case | Recommended for high-specificity screening, functional genomics, and follow-up validation | Recommended for neuronal, suspension, primary, and other hard-to-transfect cells | Recommended for transfectable mammalian cells | Recommended for transfectable mammalian cells | Highly specific lncRNA targeting |
| Transfection Requirements | DharmaFECT reagent recommended | None required | DharmaFECT reagent recommended | DharmaFECT reagent recommended | DharmaFECT reagent recommended |
| Available as SMARTpool format | ✔ | ✔ | ✔ | ✔ | ✔ |
| Available as 4 individual siRNAs | ✔ | ✔ | ✔ | ✔ | ✔ |
| Guaranteed Knockdown (SMARTpool format + 3 of 4) | ✔ (updated design enhances reliability) | ✔ | ✔ | ✔ | — |
| Sense Strand Modifications | ✔ | ✔ | ✔ | Selective application as needed | ✔ |
| Antisense Seed Region Modification | ✔ | — | ✔ | — | ✔ |
| Uptake-Enhancing Modifications | — | Modifications to support delivery | — | — | — |
| Nuclease Resistance | — | Stabilizing modifications included | — | — | — |
| Sequence Information Provided With Purchase | ✔ | ✔ | ✔ | ✔ | ✔ |
| Product Search | Search ON-TARGETplus 2.0 | Search Accell 2.0 | Search ON-TARGETplus siRNA | Search siGENOME | Search Lincode |
Pooling siRNA improves effectiveness
The SMARTpool format, which is available for ON-TARGETplus 2.0, ON-TARGETplus, Accell, Accell 2.0, siGENOME and Lincode siRNA, combines four gene-specific siRNAs into a single reagent pool. Research has shown that pooled siRNAs:
- Improve the likelihood of effective gene silencing
- Minimize sequence-specific off-targeting by lowering the relative concentration of each siRNA
- Better mimic the natural RNAi pathway
- Reduce false negatives caused by inaccessible binding sites
Experimental support
Carousel items
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Dharmacon siRNA selection guide
Choose the optimal RNA product line and format for your experiment. -
ON-TARGETplus 2.0 siRNAs
Learn how we design our off-the-shelf ON-TARGETplus 2.0 siRNAs and why periodic review of them is wise. -
Accell siRNA silencing workflow
Experiment workflow for gene silencing with Accell self-delivering siRNA. -
siRNA control recommendations
This technical note discusses the function and features of a variety of control siRNAs.
*Gene silencing guarantee applies to SMARTpool and "3 of 4 individual siRNA" formats when demonstrated to have been used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100nM siRNA). For more information regarding the siRNA knockdown guarantee, please contact tech support.
