Confidence in your knockout experiment
Dharmacon Edit-R CRISPR guide RNAs are guaranteed to edit the target gene-of-interest, so you can be confident in your knockout result – see guarantee tab for details.
Our guide RNAs are designed using a validated algorithm to achieve functional gene knockout with high specificity. By assessing phenotypes for thousands of designs, then validating our design rules in multiple assay systems, we have established standards for determining optimal knockout target sites.
Visit our application page to learn more about the Edit-R CRISPR-Cas9 gene editing system.
Flexibility to fit your workflow
We offer Dharmacon Edit-R CRISPR guide RNA in pools or as individual reagents in a variety of formats to fit many different experimental workflows.
- Synthetic single guide (sgRNA) or two part crRNA:tracrRNA reagents can be co-transfected with Cas9 mRNA or protein for DNA-free workflows.
- Lentiviral sgRNA are recommended for editing in difficult-to-transfect cells.
- All-in-one lentiviral sgRNA + Cas9 offers a single-reagent knockout workflow.
- Custom design synthetic or lentiviral guide RNAs for additional species, alternative nucleases or any target location using the CRISPR Design Tool.
Scale to meet any scope
Expand the scope of your next loss-of-function experiment. Use the Cherry-Pick Library Tool to design and order your own custom CRISPR knockout screening library of Edit-R guide RNA reagents. Or see our predefined CRISPR knockout libraries for gene families or whole genome screens.
Guide RNA reagent selection
Determining the most appropriate guide RNA format for your experiment will depend on your particular application and cell type. Use this table to select the right reagents for your specific experimental conditions.
|Synthetic crRNA||Synthetic sgRNA||Lentiviral sgRNA||Lentiviral All-in-one sgRNA|
|Guaranteed to edit your gene of interest||✔||✔||✔||✔|
|Can be custom designed for any species, nuclease or editing location||✔||✔||✔*||✔*|
|RNP compatible for electroporation||✔||✔|
|Transduce into difficult-to-edit cells that can't be electroporated||✔||✔|
|Population enrichment with antibiotic resistance markers||✔||✔|
|Population enrichment with fluorescent markers||✔**||✔**||✔|
|Single reagent workflow||✔|
*custom vector designs for use with S. pyogenes Cas9 only.
**when co-transfected with fluorescent Cas9 mRNA.
CRISPR guide RNA
Dharmacon Edit-R synthetic guide RNA & controls
Genome wide synthetic sgRNA designs guaranteed to edit your gene of interest. The design algorithm maximizes the potential for functional protein knockout while minimizing off-target editing through stringent specificity checks.
Species-specific synthetic sgRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.
Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific sgRNA.
Guaranteed to edit your target! Algorithm-optimized crRNA for genome-wide coverage of human and mouse genes. Modifications for nuclease resistance improve DNA-free editing.
Trans-activating CRISPR RNA (tracrRNA) is required for use with Edit-R synthetic crRNA to form the complex that programs Cas9 nuclease.
Dharmacon Edit-R lentiviral sgRNA & controls
Combined Cas9 and sgRNA expression for efficient gene knockout & unparalleled specificity; available as glycerol stocks and high-titer purified particles.
All-in-one lentiviral sgRNA controls to verify DNA double-strand breaks and gene editing efficiencies.
All-in-one lentiviral sgRNA constructs bioinformatically designed to not target any gene in human or mouse genomes.
Guaranteed to edit your target, algorithm-optimized sgRNA for genome-wide coverage of human or mouse genes. Provided as high-titer lentiviral particles or glycerol stocks.
Species-specific sgRNAs targeting well-characterized genes to determine the effectiveness of your gene editing conditions for maximum efficiency.
Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene-specific sgRNA.
Custom guide RNA design
Place a custom guide RNA order, or design and order your own synthetic sgRNA, crRNA, or lentiviral sgRNA with our easy-to-use interface.
Dharmacon Edit-R HDR donor template design, ordering tools & kits
Design and order a single-strand DNA donor (≤ 150 nt) for insertion, deletion, or other alteration.
Design and order a plasmid DNA donor kit for insertion of a mKate2 or EGFP fluorescent marker, or other a custom insert.
Rapidly and easily assemble a plasmid donor for HDR
Quickly and efficiently build a HDR donor plasmid
PCR components for Edit-R Plasmid Donor Kits
Cas9 nuclease sources
"CRISPR-ready" premade Cas9-expressing cell lines
Transient Cas9 nuclease for DNA-free workflows
Vector-based solutions for generating Cas9-expressing cell lines
CRISPR knockout screening libraries
Have a favorite gene list? Customize and order plates of synthetic or lentiviral guide RNAs for knockout studies in your targets of interest.
Pre-defined collections of synthetic and lentiviral guides covering popular gene families or whole genomes in pooled and arrayed format.
The Dharmacon Edit-R guarantee
We guarantee that EVERY predesigned guide RNA will provide successful editing at the target site when delivered as described in the Edit-R technical manuals.
The Edit-R guide RNA guarantee is valid when used with any wild type S. pyogenes Cas9 nuclease, including mRNA, expression plasmid, protein, or stable Cas9 expression, and Edit-R crRNAs must be used with Edit-R tracrRNA for the guarantee to apply.
Analysis of editing of the treated cell population must be shown using a T7EI or Surveyor mismatch detection assay. If successful editing is not observed for a predesigned Edit-R guide RNA while an appropriate side-by-side Edit-R positive control is successful, a one-time replacement of a different predesigned Edit-R guide RNA of the same format and quantity will be provided at no cost.
A replacement will only be approved upon discussion with our Technical Support team.
Successful editing at the DNA level does not always lead to functional gene knockout; it is recommended to test multiple guide RNAs to determine the most effective guide RNA for knockout of your target gene.
This guarantee does not extend to any accompanying experimental costs, does not apply to guide RNAs ordered via the CRISPR Design Tool, and will not be extended to the replacement guide RNA.