CRISPR guide RNA
Edit-R™ Predesigned guide RNA
Guaranteed to edit the target gene
Custom guide RNA
Design guide RNAs for use in 40+ species or with an alternative nuclease
Guide RNAs program Cas9 nucleases to cut at a specific genomic location. The design of an effective guide RNA is critical to achieving efficient gene knockout.
All Edit-R predesigned guide RNA products are designed with a validated algorithm to maximize the likelihood of functional protein knockout, not just to create a double-strand break. By assessing phenotypes for thousands of designs, then validating our design rules in other assay systems, we have established standards for determining target sites that are more likely to achieve functional knockout with high specificity.
Synthetic and lentiviral guide RNAs can also be custom designed for additional species, alternative nucleases or any specific region within a gene using the CRISPR Design Tool. For those designing guide RNA which use Staph aureus Cas9 for cleavage, Horizon recommends NEB EnGen® Sau Cas9 for your nuclease.
Guide RNAs in the CRISPR-Cas9 system
In addition to expression of the Cas9 nuclease, the CRISPR-Cas9 system requires a specific RNA molecule to recruit and direct the nuclease activity to the region of interest. These guide RNAs take one of two forms:
- A synthetic trans-activating CRISPR RNA (tracrRNA) plus a sythetic CRISPR RNA (crRNA) designed to cleave the gene target site of interest (Figure 1a)
- A synthetic or expressed single guide RNA (sgRNA) that consists of both the crRNA and tracrRNA as a single construct (Figure 1b)
Figure 1a. Illustration of Cas9 nuclease programmed by the crRNA:tracr complex cutting both strands of genomic DNA 5' of the PAM
|Figure 1b. Illustration of Cas9 nuclease programmed by the sgRNA complex cutting both strands of genomic DNA 5' of the PAM|