Cas9 nucleases

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Gene editing
Gene editing reagents
Cas9 nucleases

Cas9 nuclease solutions for every CRISPR gene editing workflow

Horizon offers a variety of Cas9 nuclease solutions for CRISPR-based gene editing experiments.
Learn more about the Edit-R™ CRISPR-Cas9 system and gene editing applications.

Cas9-expressing stable cell lines for streamlined gene editing

Shorten your next gene editing workflow with "CRISPR-ready" Cas9-expressing stable cells in a variety of popular cell types.

Fluorescent Cas9 reagents for optimization and enrichment

Simplify enrichment of Cas9 expressing cells with lentiviral or mRNA reagents that co-express either mKate2 or TurboGFP™ with Cas9.

Transient Cas9 products for DNA-free gene knockout

Reduce off-targets and protect cells from potential unwanted cellular responses with mRNA or protein reagents that permit transient Cas9 expression.

Lentiviral Cas9 for easy delivery into any cell type

Eliminate the need for cytotoxic transfection or electroporation steps with constitutive or inducible lentiviral Cas9 reagents.

Cas9 nuclease selection guide

Determining the most appropriate Cas9 nuclease reagent for your experiment is dependent on the particular application or cell type.
See the table below to determine the best format for your experiment.

	Cas9 protein	Cas9 mRNA	Lentiviral Cas9 particles
DNA-free, transient expression	✔	✔
Co-electroporate with synthetic guide RNA	✔	✔
Co-transfect with synthetic guide RNA	✔	✔
Enrich population with FACS		✔	✔
Enrich population with resistance marker			✔
Inducible expression			✔
Create stable cell lines			✔
Lentiviral transduction for cells that are difficult to transfect			✔

Order Cas9 stable cells

Cas9-expressing cells

Cas9-expressing stable cells in a variety of popular cell types - simply introduce Edit-R CRISPR guide RNA for targeted gene knockout.

Order transient Cas9 nuclease

Cas9 mRNA

Purified Cas9 mRNA for transient Cas9 nuclease expression with fluorescent options for sorting, enrichment & visualization.

Cas9 protein NLS

Purified ready-to-use Cas9 protein for rapid, RNP workflows.

Order vector-based Cas9

Lentiviral Cas9

Purified lentiviral particles or plasmid DNA for generating stable Cas9-expressing cells. Inducible promoter options available.

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Cas9 mRNA data

Cas9 expressing stable cell line supporting data — Adherent Cas9 Stable Cell Lines were plated at 10,000 cells/well and reverse transfected using DharmaFECT1 or DharmaFECT4 Transfection Reagent with synthetic sgRNA (25 nM) targeting PPIB (Cat # U-009000-01-XX). Cells were harvested 72 hours post-transfection and the relative efficiency of indel formation was assessed using trace decomposition (TIDE) compared to a non-targeting control (NTC; Cat # U-009501-01-XX). Suspension Cas9 Stable Cell Lines were electroporated using 100,000 cells/well, and Lonza buffer SE or SF, respectively, with synthetic sgRNA (5 µM) targeting PPIB (Cat # U-009000-01-XX). Cells were harvested 72 hours post-transfection and the relative efficiency of indel formation was assessed using trace decomposition (TIDE) compared ion to a non-targeting control (NTC; Cat # U-009501-01-XX).

Fluorescent Cas9 nuclease mRNA maintains editing efficiency — Fluorescent Cas9 mRNA U2OS cells were plated at 10,000 cells/well in a black 96-well plate 24 hours before transfection. At the time of transfection, EGFP Cas9 nuclease mRNA (200 ng, Cat #CAS11860) or non-fluorescent Cas9 Nuclease mRNA (200 ng, Cat #CAS11195) was co-transfected with Edit-R PPIB Synthetic crRNA Control (25 nM, Cat #U-007000-01-05) and tracrRNA (25 nM, Cat# U-002005-05) using DharmaFECT™ Duo transfection reagent (0.3 µL/well). After 24 hours, cells were imaged for EGFP fluorescence using the Nikon Eclipse Ti-S/L100 inverted microscope (A). At 72 hours after transfection, cells were harvested and gene editing was measured through a DNA mismatch detection assay (B).

Cas9 nuclease mRNA with fluorescent reporter — U2OS cells expressing a Ubiquitin-tagged EGFP were plated at 10,000 cells/well in a black 96-well plate and co-transfected with Cas9 mRNA (200 ng, Cat #CAS11859) and synthetic tracrRNA (25 nM, Cat# U-002005-05) targeting PSMD11 (25 nM, Cat #CM-085161-03), a known proteasome component, or a Non-targeting control (NTC, Cat #U-007501-xx). Increasing amounts of DharmaFECT Duo Transfection Reagent were tested per well (0.1 µL to 0.7 µL). After 24 hours, cells were imaged for mKate2 fluorescence using the In Cell Analyzer 2200. At 72 hours after transfection, cells were imaged for EGFP fluorescence resultant from proteasome knockout. Based on mKate2 fluorescence, optimal transfection conditions occur at 0.3-0.4 µL of DharmaFECT Duo per well which corresponds to an increased phenotypic effect. Above 0.4 µL of DharmaFECT Duo per well, significant cell death is observed (graph, red boxes). UT = untransfected sample.

Cas9 protein data

Synthetic guide RNAs and Cas9 nuclease protein electroporated as RNP — Guide RNAs and Cas9 protein were combined to form an RNP complex and nucleofected into CD3/CD28 stimulated CD4+ primary human T-cells. Phenotypic functional knockout was measured by FACS analysis of target gene expression 72-hours after nucleofection.

Lentiviral Cas9 data

99D7BB2F6E084F7A8CC8082A7E6A71F0 — U2OS cells were transduced at low multiplicity of infection (MOI 0.3) with Edit-R Lentiviral hCMV mKate2-Cas9 Nuclease particles (Cat #VCAS11869) so that transduced cells would have only one integration of Cas9. Cells were expanded for fluorescence activated cell sorting (FACS) where populations were sorted into negative, low, medium and high mKate2 fluorescence. These subpopulations were expanded and then plated at 10,000 cells/well in a 96-well plate. One day later, cells were transfected with tracrRNA (25 nM, Cat #U-002005-xx) and Edit-R PPIB Synthetic crRNA Control (Cat #U-007501-xx) or Edit-R MYC Predesign crRNA (Cat #CM-003282-01) using DharmaFECT 1 transfection reagent (0.3 µL/well, Cat #T-2001-01). After 72 hours, cells were imaged for mKate2 fluorescence using the In Cell Analyzer 2200 (GE Healthcare; A) and then harvested for DNA mismatch detection assay to estimate gene editing (B). High mKate2 expression can be associated with the highest levels of gene editing for both PPIB- and MYC-targeting crRNAs.

Cas9 nuclease sources

"CRISPR-ready" premade Cas9-expressing cell lines

Cas9 expressing stable cell lines

Choose from a variety of popular cell backgrounds, then simply deliver a Edit-R CRISPR guide RNA targeted to any gene for simple loss-of-function studies.

Transient Cas9 nuclease for DNA-free workflows

Cas9 nuclease mRNA

Purified Cas9 mRNA for transient Cas9 nuclease expression. Fluorescent options available for sorting, enrichment and visualization of delivery.
Cas9 nuclease protein NLS

Purified Cas9 protein ready-to-use for DNA-free workflows.

Vector-based solutions for generating Cas9-expressing cell lines

Lentiviral Cas9 nuclease reagents

Purified lentiviral particles or plasmid DNA for generation of stable Cas9 nuclease-expressing cell populations. Constitutive or inducible promoter options are available.

CRISPR guide RNA

Edit-R synthetic guide RNA & controls

Synthetic sgRNA

Genome wide synthetic sgRNA designs guaranteed to edit your gene of interest. The design algorithm maximizes the potential for functional protein knockout while minimizing off-target editing through stringent specificity checks.
- Synthetic sgRNA positive controls and detection primers
  
  Species-specific synthetic sgRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.
- Synthetic sgRNA non-targeting controls
  
  Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific sgRNA.

Synthetic crRNA

Guaranteed to edit your target! Algorithm-optimized crRNA for genome-wide coverage of human and mouse genes. Modifications for nuclease resistance improve DNA-free editing.
Synthetic tracrRNA

Trans-activating CRISPR RNA (tracrRNA) is required for use with Edit-R synthetic crRNA to form the complex that programs Cas9 nuclease.

Edit-R lentiviral sgRNA & controls

All-in-one lentiviral sgRNA + Cas9

Combined Cas9 and sgRNA expression for efficient gene knockout & unparalleled specificity; available as glycerol stocks and high-titer purified particles.

All-in-one lentiviral sgRNA positive controls and kits

All-in-one lentiviral sgRNA controls to verify DNA double-strand breaks and gene editing efficiencies.
All-in-one lentiviral sgRNA non-targeting controls

All-in-one lentiviral sgRNA constructs bioinformatically designed to not target any gene in human or mouse genomes.

Lentiviral sgRNA

Guaranteed to edit your target, algorithm-optimized sgRNA for genome-wide coverage of human or mouse genes. Provided as high-titer lentiviral particles or glycerol stocks.

Lentiviral sgRNA positive controls

Species-specific sgRNAs targeting well-characterized genes to determine the effectiveness of your gene editing conditions for maximum efficiency.
Lentiviral sgRNA non-targeting controls

Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene-specific sgRNA.

Custom guide RNA design

CRISPR Design Tool

Place a custom guide RNA order, or design and order your own synthetic sgRNA, crRNA, or lentiviral sgRNA with our easy-to-use interface.

Edit-R HDR donor template design, ordering tools & kits

HDR Donor Designer - oligo

Design and order a single-strand DNA donor (≤ 150 nt) for insertion, deletion, or other alteration.
HDR Donor Designer - plasmid

Design and order a plasmid DNA donor kit for insertion of a mKate2 or EGFP fluorescent marker, or other a custom insert.
HDR plasmid donor kits

Rapidly and easily assemble a plasmid donor for HDR
HDR plasmid donor components

Quickly and efficiently build a HDR donor plasmid
HDR plasmid donor primers

PCR components for Edit-R Plasmid Donor Kits

Cas9 nucleases

Purified and lentiviral expressed Cas9 nuclease solutions for CRISPR gene editing experiments.

Cas9 nuclease solutions for every CRISPR gene editing workflow

Cas9-expressing stable cell lines for streamlined gene editing

Fluorescent Cas9 reagents for optimization and enrichment

Transient Cas9 products for DNA-free gene knockout

Lentiviral Cas9 for easy delivery into any cell type

Cas9 nuclease selection guide

Order Cas9 stable cells

Order transient Cas9 nuclease

Order vector-based Cas9

Not sure where to start?

Supporting data

Cas9 mRNA data

Efficient indel formation using Edit-R synthetic sgRNA in Cas9-expressing stable cell lines

Fluorescent Cas9 nuclease mRNA maintains editing efficiency

Fluorescent Cas9 nuclease mRNA indicates optimal transfection conditions

Cas9 protein data

Synthetic guide RNAs and Cas9 nuclease protein electroporated as RNP

Lentiviral Cas9 data

Fluorescent lentiviral Cas9 nuclease enables enrichment for high expression and improved CRISPR-Cas9 gene editing efficiency

Reagents & controls

Cas9 nuclease sources

CRISPR guide RNA

Edit-R synthetic guide RNA & controls

Guarantee

The Edit-R guarantee