Gene editing

Understand disease mechanisms at the genomic level


Gene editing refers to the process of changing regions of cellular DNA. The most common gene editing techniques involve inactivating a gene’s function (knockout), introducing or correcting a SNP mutation, or adding a reporter tag to an endogenous gene (knock-in). These changes are permanent and heritable, resulting in a newly engineered cell line.
With our Dharmacon™ Edit-R™ CRISPR-Cas9 reagents, researchers can make targeted, specific edits to any gene in practically any cell type. Additionally, Horizon offers a wide selection of pre-made edited cell lines and expert genome engineering services.

Dharmacon Edit-R gene editing reagents

Guaranteed to edit your target gene

Algorithm designed CRISPR guide RNA to maximize functional protein knockout and minimize off-target effects.

Also find numerous Cas9 nuclease options, positive and non-targeting controls, and donor templates for HDR-mediated knock-in applications.

Engineered cell models

Ready-to-go or tailored for individual needs

Find 1000's of validated knockout models, point mutations in cancer-relevant cell models, and "CRISPR-ready" stable Cas9 or dCas9-VPR expressing cell lines.

Or get in touch with our experts to design the exact knockout, Knock-in or iPSC required for your drug discovery pipeline. Your edit, our expertise.

CRISPR-Cas9 genome editing overview

App note: CRISPR knockout in iPSCs

Highly specific and efficient gene knockout See how Dharmacon Edit-R reagents are used to generate functional protein knockouts in human induced pluripotent stem cells.

Gene editing workflow guide

Planning a gene knockout or knock-in?  This workflow guide will assist you in selecting the best Dharmacon genome engineering tools for your application.

CRISPR screening libraries

Accelerate functional genomic screens with CRISPR libraries Available custom designed or pre-defined in pooled and arrayed formats.

CRISPR & Cas9 All-in-one vector!

Single-transfection solution for a simplified workflow

Express both Cas9 nuclease and a gene specific guide RNA with your choice of fluorescent reporter or antibiotic resistance for easy selection.

Knock-in your edit 

Create a SNP or knock-in a reporter gene Design templates for precise genomic insertion using the HDR pathway.
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CRISPR Design Tool

Algorithm guided custom CRISPR guide RNA design. Design guide RNA for use in over 40 species or with any nuclease.

Dharmacon guarantee

Peace of mind that your editing experiment will be successful All predesigned guide RNAs are fully guaranteed to edit the target gene, ensuring the highest likelihood of a successful knockout experiment.