Silence your target gene
To achieve guaranteed target gene knockdown, simply combine Accell siRNA stock solution with Accell siRNA delivery media to a recommended 1µM working concentration. Then add Accell delivery mix directly to cells and incubate for 72 hours.
Delivery may be inhibited by the presence of BSA in serum. Optimization with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended. Full-serum media may be added back after 48 hours of incubation
mRNA silencing is achieved by 72 hours, with optimal protein knockdown observed around 96 hours.
Simple but effective siRNA delivery
Cellular uptake of Accell siRNA is shown to increase over time with extended incubation. Cerebellar sections (250 μm) were prepared, cultured, and incubated for 3 hours (left) and 72 hours (B) with Accell Red Non-targeting control (NTC) siRNA before inspection by microscopy.
Increased fluorescence detected in the left panel demonstrates successful Accell siRNA delivery.
Highly efficient protein knockdown
Target gene expression is shown to decrease over time with extended incubation, with optimal gene silencing observed at 96 hours.
Cultured brain slices were assessed following incubation with Accell siRNA targeting PPIB and GAPDH or Non-targeting controls (NTC) at three time points (48, 72, 96 hours) and the level of remaining protein was evaluated using Western blot analysis (using Actin as loading control).
See how Accell siRNA achieves effective and specific target gene knockdown in multiple difficult cell types, including SH-SY5Y (neuroblastoma), Jurkat (T-cells), and primary neurons.
Repeated application of Accell siRNA allows for extended duration gene knockdown with only minimal effects on cell viability and the innate immune response.
Accell siRNA is used in brain tissue slices for highly specific and effective target gene interference.