Accell™ siRNA by Dharmacon™ is uniquely modified to facilitate passive uptake into the cell – eliminating the need for viral vectors, cytotoxic transfection or electroporation techniques. Simply add one reagent to silence any human, mouse or rat gene, in nearly any cell line.
Accell benefits
- Single reagent – no viral vector, transfection or electroporation required
- Easy delivery – simply add the appropriate amount to your cells
- Guaranteed gene silencing – all Dharmacon siRNA are guaranteed to knockdown the target gene
Ideal for
- Difficult-to-transfect cell types – primary, neuron, immune cells & iPSCs
- Gene knockdown – simplified workflow for guaranteed results
- Extended-duration silencing – low toxicity enables multiple dosing up to 30 days
The power of Accell siRNA lies in the simplicity of it's workflow
To achieve guaranteed target gene knockdown, simply combine Accell siRNA stock solution with Accell siRNA delivery media to a recommended 1µM working concentration. Then add Accell delivery mix directly to cells and incubate for 72 hours.
Delivery may be inhibited by the presence of BSA in serum. Optimization with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended. Full-serum media may be added back after 48 hours of incubation mRNA silencing is achieved by 72 hours, with optimal protein knockdown observed around 96 hours.
Accell gene silencing workflow
Accell gene silencing workflow
(1) Combine Accell siRNA with Accell delivery media (or other low- or no-serum media) (2) Add Accell delivery mix directly to cells and incubate for 72 hours.
Accell gene silencing workflow
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Accell siRNA
Find Accell siRNA for simple, guaranteed knockdown of any human, mouse or rat gene
Accell controls
Positive and non-targeting controls for gene knockdown experiments using Accell siRNA
Find whole genome and predefined pathway or gene family screening libraries of Accell siRNA
Helpful Resources
Innovative technology that enables RNAi in difficult to transfect cells - Poster
Investigations at Dharmacon have led to the development of an innovative molecule for delivery in a wide variety of cell types. These modified siRNAs have been found to effectively silence target genes in cell types that are typically difficult to transfect using standard delivery methods
Gene Silencing Workflow with Accell siRNA - Application Note
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Accell siRNA - Achieving Long-term Gene Silencing - Application Note
Long-term gene knockdown is important when understanding the gene function, but lentiviral approaches are not always possible. In this document we demonstrate how to get 30 day gene down regulation by repeated application of Accell modified siRNA without affecting the viability of the cells
A Novel ex vivo Application of RNAi for Neuroscience - Application Note
Demonstrates use of Accell siRNA in ex vivo culture as a means of bridging the gap between in vitro cell cultures and in vivo models for neuronal molecular pathway research
Simple but effective siRNA delivery
Highly efficient protein knockdown