New: Reagents for the Pin-point base editing platform are now available in both Adenine base editing (ABE) and Cytosine base editing (CBE) configurations. View the product pages on the right for more details.
Revvity's Pin-point base editing platform offers a unique and powerful approach for safe and more effective complex genetic engineering. This page is dedicated to our Pin-point base editing reagents that are available for research use only. The Pin-point™ base editing platform technology is also available for clinical or diagnostic study and commercialization under a commercial license from Revvity. To learn more about potential licensing and service options please visit revvity.com
The modular Pin-point™ base editing system facilitates highly efficient and precise nucleotide conversion with the potential for multiplex gene editing capabilities. The modularity of the system means we can offer a customizable, off-the-shelf system for base editing.
Pin-point base editing technology
Revvity's Pin-point™ base editing technology is a flexible, three-part system that uses an engineered guide RNA to bring all the pieces of the editing machinery together on-target in the genome.
A nickase Cas9 (nCas9) is guided to a specific DNA sequence by a modified guide RNA (gRNA). This gRNA includes an aptamer "handle", which acts like a docking site to recruit the editing enzyme (such as a deaminase) via a matching binding protein.
By placing the recruitment function on the gRNA rather than permanently attaching the enzyme to Cas9, the system allows for greater flexibility. This means you can mix and match components, fine-tune activity, and even recruit multiple functions at once for more advanced genome engineering applications such as CAR-T cells generation.
System components
- Guide RNA (gRNA) with built-in aptamer "handle"
- Nickase Cas9 (nCas9) for target DNA recognition and strand nicking
- Effector protein (e.g., deaminase) fused to an aptamer-binding protein for recruitment
Benefits of Pin-point base editing platform include:
MODULAR SYSTEM for
optimized research
HIGH EFFICIENCY
gene editing platform
MULTIPLEX EDITING
across several targets
IMPROVED SAFETY over
standard CRISPR-Cas9
VERSATILE
TECHNOLOGY for targeted editing
VALIDATED
PERFORMANCE in T cells and iPSCs
Read publications about the Pin-point base editing platform:
Order Pin-point base editing reagents
Custom Pin-point Synthetic sgRNAs
Use our custom design tool to create base editing sgRNAs for gene knockout or enter in your own 20nt spacer sequence to introduce targeted point mutations.
Pin-point Base Editing mRNA
The Pin-point base editing mRNA system allows for precisely directed point mutation edits without inducing double stranded breaks or the need for homology directed repair.
Pin-point Synthetic sgRNA Validated Controls
Synthetic sgRNA controls to verify optimal parameters for precise gene editing without inducing DNA double-strand breaks.
Pin-point Synthetic sgRNA Non-targeting Controls
Non-targeting synthetic controls for evaluation of Pin-point base editing. Bioinformatically designed not to target any gene in the human genome.
Learn more in our base-editing reagents focused application notes
Complex genome engineering with the Pin-point base editing system, even in sensitive cell types
An application note demonstrating complex genome engineering with the Pin-point base editing system.
Guidance for using unmodified versus 5-methoxyuridine (5moU) modified mRNAs with chemically synthesized sgRNAs
Optimized stem and immune cell editing with the Pin-point™ base editing platform
Designing and evaluating single guide RNAs for introducing protein knockout with the Pin-point base editing platform
In this application note we’ll walk you through designing a guide RNA spacer sequence for a base editing experiment and demonstrate our approach to evaluating several candidate guide RNAs to identify the best one for generating a functional knockout.