Complex genome engineering with the Pin-point base editing system, even in sensitive cell types
An application note demonstrating complex genome engineering with the Pin-point base editing system.
Pin-point Base Editing Reagents
Accelerate therapeutic development with Revvity's Pin-point™ base editing reagents.
The modular Pin-point base editing platform combines engineered guide RNA, nickase Cas9, and a deaminase enzyme to deliver precise single-nucleotide edits in sensitive cell types, including T cells and iPSCs, without double-strand breaks. The system's modularity allows customizable configurations for both adenine (ABE) and cytosine (CBE) base editing.
With validated performance in T cells, iPSCs, and HSPCs, the Pin-point base editing platform delivers improved safety over standard CRISPR-Cas9 with multiplex editing capabilities.
Pin-point base editing reagents are available for research use only and are not for diagnostic use or direct administration into humans or animals. The Pin-point™ base editing platform technology is available for clinical or diagnostic study and commercialization under a commercial license from Revvity.
Pin-point base editing reagents
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Pin-point base editing mRNA
The Pin-point base editing mRNAs come in ABE and CBE configurations for co-electroporation with Pin-point synthetic sgRNAs. -
Custom Pin-point synthetic sgRNAs
Use our custom design tool to create base editing sgRNAs for gene knockout or enter in your own 20nt spacer sequence to introduce targeted point mutations. -
Pin-point synthetic sgRNA validated controls
Synthetic sgRNA controls to co-electroporate with Pin-point base editing mRNAs to verify optimal parameters for base editing. -
Pin-point synthetic sgRNA non-targeting controls
Non-targeting synthetic controls for determination of baseline cellular responses in Pin-point base editing experiments.
Benefits of the Pin-point base editing platform
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Precise single-nucleotide editing without double-strand breaks
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Validated performance and verified reagents for use in T cells, iPSCs, and HSPCs
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Improved safety over standard CRISPR-Cas9
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Modular system for optimized research
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Multiplex editing across several targets
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Versatile technology for targeted editing
Pin-point base editing technology
Revvity's Pin-point base editing technology is a flexible, three-part system that uses an engineered guide RNA to bring all the pieces of the editing machinery together on-target in the genome.
A nickase Cas9 (nCas9) is guided to a specific DNA sequence by a modified guide RNA (gRNA). This gRNA includes an aptamer "handle", which acts like a docking site to recruit the editing enzyme (such as an ABE or CBE deaminase) via a matching binding protein.
By placing the recruitment function on the gRNA rather than permanently attaching the enzyme to Cas9, the system allows for greater flexibility. This means you can mix and match components, fine-tune activity, and even recruit multiple functions at once for more advanced genome engineering applications such as CAR-T cells generation.
System components
- Guide RNA (gRNA) with built-in aptamer "handle"
- Nickase Cas9 (nCas9) for target DNA recognition and strand nicking
- Effector protein (e.g., ABE or CBE deaminase) fused to an aptamer-binding protein for recruitment
Revvity's Pin-point base editing platform
The modular Pin-point base editing system facilitates highly efficient and precise nucleotide conversion with the potential for multiplex gene editing capabilities. The modularity of the system means we can offer a customizable, off-the-shelf system for base editing.
Reagents for the Pin-point base editing platform are now available in both Adenine base editing (ABE) and Cytosine base editing (CBE) configurations. View the product pages for more details.
To learn more about potential licensing and service options please visit revvity.com.
Application notes: See Pin-point base editing in action
Guidance for using unmodified versus 5-methoxyuridine (5moU) modified mRNAs with chemically synthesized sgRNAs
Optimized stem and immune cell editing with the Pin-point base editing platform
Designing and evaluating single guide RNAs for introducing protein knockout with the Pin-point base editing platform
In this application note we’ll walk you through designing a guide RNA spacer sequence for a base editing experiment and demonstrate our approach to evaluating several candidate guide RNAs to identify the best one for generating a functional knockout.
Publications: Read published research on Pin-point technology
Don't break my strands
Published in the Medicine Maker
Base editing: a strong contender in cell and gene therapy
Published in GEN
CRISPR 2.0: base editing in the groove
Published in GEN
Base editing: a new player in the cell and gene therapy space
Published in American Pharmaceutical Review
