Protocol
Ethanol precipitation of RNA oligonucleotides
This protocol is for Ethanol Precipitation of RNA Oligonucleotides.
Custom Antisense Oligonucleotide Synthesis
Custom ASOs with flexible modifications to meet your needs
Design custom antisense oligonucleotides (ASOs; 15-35 nucleotides) with the chemical modifications, synthesis scale, and purification options your experiments demand. From individual oligos to bulk orders, our synthesis tool makes it simple to get exactly what you need.
Supported modifications include but are not limited to phosphorothioate linkages, 2'-O-methyl bases, locked nucleic acids, and MOE (methoxyethyl), applied across single-strand DNA, RNA, and RNA-DNA chimeras. Order at 0.05 μmol or 1.0 μmol scale, with HPLC purification available. Additional modifications are available on request.
For oligos outside the 15-35 nucleotide range, see our custom RNA and custom DNA ordering options.
Order custom antisense oligos
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Individual ASOs
Custom synthesis of 15-35 bases at either 0.05 μmol or 1.0 μmol scale, with the option of HPLC purification.
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Multiple ASOs
Use this online tool to order multiple custom ASOs with consistent characteristics
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Other ASO or DNA synthesis solutions
Don't see what you need? Connect with our scientific support team for many more options for oligo customization.
Why choose custom ASO synthesis?
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Individual or batch ordering
Order individual ASOs or place bulk orders with consistent characteristics (scale, purification, and modifications).
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Flexible modifications
Choose from phosphorothioate linkages, 2'-O-methyl, LNA, MOE, and more to enhance stability and binding affinity. A full list of modifications available on our web ordering tools can be found at our Bases and Modifications page.
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Proven expertise
Backed by 30+ years of oligo synthesis experience and trusted by researchers worldwide.
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Confident results
All oligos made by the Dharmacon Reagents team are examined by mass spectrometry. Additional ultra-HPLC analytical data and mass spec traces are available on request as an additional service.
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Custom support available
Request modifications or customized purification and processing beyond the standard catalog or get guidance from our team. Contact our scientific support team.
Ordering custom ASOs
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What is an ASO?
Learn more about antisense oligos (ASOs) on our application page. -
How do I order individual ASOs?
To order individual ASOs, use our online tool to enter your sequence (15-35 nucleotides), select the synthesis scale (0.05 μmol or 1.0 μmol), choose modifications, and specify purification options. The tool will provide an instant quote. -
What modifications are available for custom ASOs?
We support a wide range of chemical modifications including phosphorothioate linkages, 2'-O-methyl, locked nucleic acids (LNA), and methoxyethyl (MOE). Browse our full modifications catalog or contact us for custom requests. -
Can I order multiple ASOs with the same specifications?
Yes, use our bulk ordering tool to upload a batch of sequences with identical characteristics (scale, purification, modifications). -
What if I need oligos outside the 15-35 nucleotide range?
For sequences shorter or longer than 15-35 bases, check out our custom RNA and custom DNA synthesis options. -
How do I get guidance or support?
Our Scientific Support team is available to provide personalized assistance, whether you need help with modification selection, yield estimates, or have a custom request. Contact us for expert guidance.
Featured technical documents
Protocol
Reverse phase cartridge desalting of RNA oligonucleotides
Protocol for desalting RNA oligos using reverse phase cartridges, including protocol for cartridge equilibration, washing, and eluting from cartridge.
Protocol
TCEP reaction for thiol-modified siRNA/RNA oligonucleotides
Protocol to deprotect (reduce) oxidized thiol-modified siRNA and RNA oligonucleotides.
Recommended reading
Publication list highlighting Dharmacon reagent development and collaborations across CRISPR-Cas9, RNAi and oligo synthesis.
Additional information
Convert your sequence from other common oligo shortcodes with our webtool
Need help entering your sequence using our oligo shortcode?
- Try using our Dharmacon Oligo Sequence Converter.
Convenient webtools to simplify your research
- Access our Ordering and calculation tools page for convenient webtools to support everything from designing your own siRNA or CRISPR guide against any organism to analyzing your PCR primers.
Revvity's adherence with the United States framework for Nucleic Acid synthesis screening
- Revvity's Dharmacon Reagents team adheres to the guidelines in the United States Framework For Nucleic Acid Synthesis. For more information please see our self-attestation.
