CRISPR guide RNAs program Cas9 nuclease to cut genomic DNA at a specific location. Once the double-strand break (DSB) occurs, the mammalian cell utilizes endogenous mechanisms to repair the DSB. In the presence of a donor DNA, either a ssDNA oligo or a plasmid donor, the DSB can be repaired precisely using HDR resulting in a desired genomic alteration (insertion, removal, or replacement).
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- Knock-in a SNP alteration in iPSCs - Application Note
- Knock-in a fluorescent tag into iPSCs using Edit-R reagents - Application Note
- Optimized HDR-mediated fluorescent protein knock-in in K-562 cells - Application Note
- Fluorescent tagging of an endogenous gene by homology-directed repair - Application Note