Protocol - Ethanol precipitation of RNA oligonucleotides
This protocol is for Ethanol Precipitation of RNA Oligonucleotides.
Custom DNA Synthesis
Custom DNA oligos to power your research applications.
Design custom single-strand DNA oligos with the synthesis scale, purification options, and modifications your experiments require. Whether you need PCR primers, HDR donor templates, NGS adapters and indices, or stabilized oligos for specialized applications, our synthesis tool makes ordering straightforward.
Custom DNA synthesis supports oligos up to 150 bases in length, ordered at 0.05 nmol or 1.0 µmol scale, with HPLC purification available. Multiple stabilizing modifications are supported to meet research-specific needs, including applications that require DNA donor oligos for CRISPR-Cas9 HDR or primers and templates.
If you need to order multiple custom DNA oligos with the same characteristics (scale, purification, 5’ modification), use our online tool for bulk orders.
Reagents
Order custom DNA oligos
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Individual DNA oligos
Custom synthesis of up to 150 bases at either 0.05 µmol or 1.0 µmol scale, with the option of HPLC purification. -
Multiple DNA oligos
Use this online tool to order multiple custom DNA oligos with consistent characteristics. -
Other custom DNA needs
Don’t see what you need? Connect with our scientific support team for many more options for oligo customization.
Why choose custom DNA synthesis?
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Individual or bulk ordering:
Order individual DNA oligos or place bulk orders with consistent characteristics (scale, purification, and 5' modifications).
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Flexible modifications:
Choose from multiple scales (0.05 µmol or 1.0 µmol) and HPLC purification, with stabilizing modifications available to meet your research needs. A full list of modifications available on our web ordering tools can be found at our Bases and Modifications page.
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Proven expertise:
Backed by 30+ years of oligo synthesis experience and trusted by researchers worldwide.
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Confident results:
All oligos made by the Dharmacon Reagents team are examined by mass spectrometry. Additional ultra-HPLC analytical data and Mass Spec data are available on request as an additional service.
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Custom support available:
Request modifications or customized purification and processing beyond the standard catalog or get guidance from our team. Contact our Scientific support team today.
Ordering custom DNA oligos
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How do I order individual DNA oligos?
To order individual DNA oligos, use our online tool to enter your sequence (up to 150 bases), select the synthesis scale (0.05 µmol or 1.0 µmol), choose any 5' modifications, and specify purification options. The tool will provide an instant quote. -
What modifications are available for custom DNA oligos?
We support multiple stabilizing modifications to meet research-specific needs. Contact our Scientific support team for detailed information or custom requests. -
Can I order multiple DNA oligos with the same specifications?
Yes, use our bulk ordering tool to upload a batch of sequences with identical characteristics (scale, purification, and 5' modifications). -
How do I get design guidance or support?
Our Scientific Support team is available to provide personalized assistance, whether you need help with modification selection, yield estimates, or have a custom request. Contact us for expert guidance.
Supporting data
Gene editing utilizing HDR at the EMX1 locus with multiple DNA donor oligo options.
A U2OS integrated Cas9 cell line was plated at 10,000 cells per well (96-well plate) and transfected with synthetic crRNA:tracrRNA and DNA donor oligo using DharmaFECT DUO. HDR insertion efficiency at the EMX1 locus was analyzed 72 hours after transfection. Percent HDR was calculated using an RFLP assay with NheI restriction digestion of EMX1 PCR amplicons.
Convert your sequence from other common oligo shortcodes with our webtool
Need help entering your sequence using our oligo shortcode? Try using our Dharmacon Oligo Sequence Converter.
Additional information
Considerations for successful HDR gene editing
Read our guidelines for setting up homology-directed repair (HDR) gene editing experiments
Revvity's adherence with the United States Framework For Nucleic Acid Synthesis Screening
Revvity's Dharmacon Reagents team adheres to the guidelines in the United States Framework For Nucleic Acid Synthesis. For more information please see our self-attestation.Featured resources
Protocol - TCEP reaction for thiol-modified siRNA/RNA oligonucleotides
Protocol to deprotect (reduce) oxidized thiol-modified siRNA and RNA oligonucleotides.
Recommended reading
Publication list highlighting Dharmacon reagent development and collaborations across CRISPR-Cas9, RNAi, and oligo synthesis.
SDS (NA) RNA Oligo
North American standard Safety Data Sheet for RNA Oligo
SDS (EU) RNA Oligo
European Union standard Safety Data Sheet for RNA Oligo
