Cherry pick libraries - synthetic

When Horizon pre-defined RNAi Screening Libraries don't match your experimental requirements, you can make your own custom library with ease, speed and economy.

You are able to cherry-pick from our premier siRNA, microRNA, Edit-R CRISPR (KO) crRNA and synthetic sgRNA,  CRISPRmod CRISPRa crRNA and synthetic sgRNA and CRISPRmod CRISPRi synthetic sgRNA reagents for human and mouse reagents to create the ideal collection for your particular needs.

Here's how:

1. Load gene identifiers for your desired products
• NCBI Gene ID or Gene Symbol
• miRBase mature name or Accession
• Dharmacon product catalog numbers
2. Review the results for accuracy and product availability
3. Select the product lines and product formats to include on the plates
4. Configure the library plates for quantity, controls, replicates and layout
5. Add to cart and check out!

Which siRNA is right for you?

Horizon offers four complete pre-designed product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.

	Cost-effective, efficient silencing	High specificity for reduced off-targets + efficient silencing	Highly specific knockdown of long noncoding RNA (lncRNA)	Target silencing in difficult-to-transfect cells
	siGENOME siRNA	ON-TARGETplus siRNA	Lincode siRNA	Accell siRNA
Pre-designed for Human, Mouse and Rat protein-coding genes
Pre-designed for Human and Mouse long noncoding RNA (lncRNA)
Recommended for transfectable mammalian cells in culture
Recommended for neuronal, suspension, primary and other difficult-to-transfect cells
Recommended transfection reagent	DharmaFECT transfection reagent	DharmaFECT transfection reagent	DharmaFECT transfection reagent	None required
Available as SMARTpool reagent
Available as four individual siRNAs
Guaranteed knockdown by SMARTpool and 3 of 4 siRNAs
Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake	Selective application when thermodynamic analysis indicates it necessary for favorable antisense RISC loading
Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity
Modifications to facilitate cellular uptake without separate transfection reagents
Stabilizing modifications to prevent nuclease-mediated degradation
Sequence information provided with purchase

• SMARTpool siRNA reagent
  A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity. siGENOME and ON-TARGETplus SMARTpool are guaranteed† to silence by 75% or better.

• Individual siRNA
  Select 1, 2, 3 or 4 individual siRNAs† per gene.

• microRNA Mimics and Hairpin Inhibitors
  Available for human and mouse microRNAs in miRBase v.21

• Pool of crRNA or sgRNA
  A mixture of 4 Edit-R CRISPR (KO) crRNA provided as a single reagent.
  A mixture of 4 CRISPRmod CRISPRa synthetic crRNA provided as a single reagent.
  A mixture of 3 Edit-R CRISPR (KO) synthetic sgRNA provided as a single reagent.
  A mixture of 3 CRISPRmod CRISPRi synthetic sgRNA provided as a single reagent.
  May not be combined with RNAi products on the same plate.

• Individual crRNA or sgRNA
  Select up to 5 individual Edit-R CRISPR (KO) crRNA per gene.
  Select up to 4 individual CRISPRmod CRISPRa synthetic crRNA per gene.
  Select up to 3 individual Edit-R CRISPR (KO) synthetic sgRNA per gene.
  Select up to 3 individual CRISPRmod CRISPRi synthetic sgRNA per gene.
  May not be combined with RNAi products on the same plate.

†Our siRNA knockdown guarantee:
siGENOME and ON-TARGETplus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when demonstrated to have been used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100nM siRNA).

Note: Most siGENOME and ON-TARGETplus siRNA products are highly functional at 5 to 25nM working concentration.

• Human siRNA Libraries
  Choose from three siRNA product lines, including two whole-genome collections, to find a SMARTpool or individual siRNA library suitable for your discovery efforts.

• Mouse siRNA Libraries
  Select ON-TARGETplus for unsurpassed specificity. Select siGENOME libraries for the most up-to-date mouse siRNAs available for optimal gene target coverage.

• Lincode siRNA Libraries
  Choose a collection of Human or Mouse siRNAs based on RefSeq lncRNA genes with NR_ Accession numbers.

• miRIDIAN microRNA Libraries
  Mimic and Hairpin Inhibitor libraries are available for human, mouse, and rat microRNAs. Provided in 96-well plates for high-throughput phenotypic screening. 

• Edit-R CRISPR (KO) synthetic sgRNA libraries
  Select arrayed collections of pre-designed synthetic sgRNA for loss-of-function screening across the human genome and gene families

• CRISPRmod CRISPRa synthetic sgRNA arrayed libraries
  Select arrayed collections of pre-designed synthetic sgRNA for gain-of-function screening across the human genome and gene families