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Power and flexibility of Edit-R CRISPR design tool
Horizon’s Edit-R CRISPR design tool generates guide RNAs for straightforward knockout experiments, as well as custom site-specific guides that target user-defined regions of a gene
By assessing phenotypes for thousands of designs, we established standards for identifying target sites that are more likely to achieve functional protein knockout while retaining high levels of specificity
Supports 30+ species, alternative nucleases beyond standard SpCas9, and can design guides not just in the protein-coding region but also for microRNAs, long non-coding RNAs, and intronic regions
Custom guide designs can be made as a synthetic guide or packaged into any of our lentiviral vectors, including the All-in-one vector, which combines SpCas9 sequence and a gene-specific guide RNA for a single, ready to use reagent.