RNA sequencing (RNA-seq) of oncogenic fusions is becoming increasingly popular in cancer research and diagnosis. Here, we talk about the benefits of using RNA in fusion detection workflows, the challenge of workflow variability and how to be confident of your assay results.
The high-throughput and increasingly affordable nature of next-generation sequencing (NGS) has led to its expanded use in routine clinical procedures. The relative simplicity of targeted enrichment cancer panels (available from a number of commercial providers) allows routine laboratories to simultaneously analyze the coding (exonic) regions of multiple cancer-related key genes. Combine this with the statistic diagnostic testing now influencing over 70 percent of all health care decisions1, the setting up or transitioning to NGS-based oncology panels for labs has never been more important.
A complete list of all our most frequently asked questions relating to HAP1 knockout cell lines. If you want to know how they're generated, how they're validated or how to find out if they're right for you - this is a great place to start.
Much information about the role of specific genes in fundamental biological processes and the onset and progression of genetic disease has been gleaned by researchers having the ability to selectively alter the genomic composition of individual genes and study the consequences. This approach enables researchers to observe the effects of a specific mutation, SNP or deletion in combination with the added layers of regulation present within the cell, including post-translational modification, epigenetic changes associated with chromatin structure, and transcriptional mechanisms.
Following transfection of CRISPR reagents, cells will need to be single cell diluted to obtain a clonal population. In this blog, we review the method used in-house.
It’s hard to keep up with the rapidly expanding world of CRISPR, and it’s starting to feel like CRISPR screens are being published every week, taking the technique from the cutting edge to the mainstream. If you’d like to understand a bit more about CRISPR screens, here’s a number of fantastic publications that have really moved this technology forward.
A review of the main 5 tips to choose and use a cell line. We highlight the importance of checking for authenticity, and contamination.
Choose the right Edit-R™ tools for your application.
DharmaFECT™ transfection reagents provide multiple formulations for your specific application
Synthetic crRNA Positive Controls and DNA Mismatch Detection Primers