Ubiquitination pathway

The ubiquitination pathway has been implicated in a number of genetic disorders and diseases including cancer and neurological diseases which result from the build-up of protein aggregates such as; Alzheimer’s disease, Parkinson’s disease and Huntingdon’s disease. This collection has been generated in close collaboration with Prof. Steve Jackson (Gurdon Institute, Cambridge, United Kingdom).

Deubiquitination image

Experimental validation

Deubiquitination of FANCD2 by USP1 is impacted in a USP1 knockout cell lines.

Cells were washed with PBS, lysed in Frackelton buffer (10 mM Tris/HCl pH 7.5, 50 mM NaCl, 30 mM sodium pyrophosphate, 1 % Triton X-100, 50 mM NaF and protease inhibitors).

They were analyzed by SDS-PAGE and Western blotting using anti-FANCD2 (sc-20022 from Santa Cruz). HAP1 wild-type cells were compared to cells bearing a frameshift mutation in USP1.

 
 
 
 
Examples of popular knockout cell lines for deubiquitinating enzymes genes
ATXN3 COPS6 CYLD JOSD2 OTUD3 OTUB1
OTUD5 PAN2 TDG UNG USP15 USP11
USP18 USP20 USP24 USP28 USP34 USP31
USP4 USP45 USP48 USP54 ZRANB1 USP8
BRCC3 COPS7A EIF3H MPND OTUD4 OTUB2
OTUD6B STAMBP UCHL1 USP1 USP16 USP13
USP19 USP21 USP25 USP3 USP38 USP32
USP40 USP46 USP49 USP6 USP9X
COPS5 COPS7B JOSD1 MYSM1 OTUD1
OTUD7B STAMBPL1 UCHL3 USP10 USP14
USP2

USP22

USP27X USP30 USP33
USP42 USP47 USP53 USP7 VCPIP1