Ubiquitination pathway
The ubiquitination pathway has been implicated in a number of genetic disorders and diseases including cancer and neurological diseases which result from the build-up of protein aggregates such as; Alzheimer’s disease, Parkinson’s disease and Huntingdon’s disease. This collection has been generated in close collaboration with Prof. Steve Jackson (Gurdon Institute, Cambridge, United Kingdom).
Deubiquitination of FANCD2 by USP1 is impacted in a USP1 knockout cell lines.
Cells were washed with PBS, lysed in Frackelton buffer (10 mM Tris/HCl pH 7.5, 50 mM NaCl, 30 mM sodium pyrophosphate, 1 % Triton X-100, 50 mM NaF and protease inhibitors).
They were analyzed by SDS-PAGE and Western blotting using anti-FANCD2 (sc-20022 from Santa Cruz). HAP1 wild-type cells were compared to cells bearing a frameshift mutation in USP1.
Examples of popular knockout cell lines for deubiquitinating enzymes genes
| ATXN3 | COPS6 | CYLD | JOSD2 | OTUD3 | OTUB1 |
| OTUD5 | PAN2 | TDG | UNG | USP15 | USP11 |
| USP18 | USP20 | USP24 | USP28 | USP34 | USP31 |
| USP4 | USP45 | USP48 | USP54 | ZRANB1 | USP8 |
| BRCC3 | COPS7A | EIF3H | MPND | OTUD4 | OTUB2 |
| OTUD6B | STAMBP | UCHL1 | USP1 | USP16 | USP13 |
| USP19 | USP21 | USP25 | USP3 | USP38 | USP32 |
| USP40 | USP46 | USP49 | USP6 | USP9X | |
| COPS5 | COPS7B | JOSD1 | MYSM1 | OTUD1 | |
| OTUD7B | STAMBPL1 | UCHL3 | USP10 | USP14 | |
| USP2 |
USP22 |
USP27X | USP30 | USP33 | |
| USP42 | USP47 | USP53 | USP7 | VCPIP1 |
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