cDNAおよびORFクローン

Mammalian cDNAs

Derived from mRNA and including 5’ and 3’ UTRs, our cDNA collections are ideal for overexpressing a gene in the context of native regulation.

• Mammalian Gene Collection (MGC) Clones

  Completed by the NIH in 2009, the MGC is now recognized as the gold standard for full-length, fully sequenced cDNA collections.

Mammalian ORFs

With both 5’ and 3’ UTRs removed, open reading frames (ORFs) provide a shortcut to protein expression.

• Precision LentiORFs

  Precision LentiORFs are expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.

• ORFeome Collaboration Collection

  ORFeome Collaboration (OC) clones are fully-sequenced ORFs in Gateway recombinational entry vectors. OC clones are available with and without stop codons.

• CCSB Human ORFeome

  This Gateway adapted human ORF collection is ideal for easily moving thousands of open reading frames into your choice of protein expression systems.

• Human ORFeome V8.1 Library

  This fully-sequenced collection of Gateway-adapted ORFs represents the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.

• CCSB-Broad Lentiviral Expression Library

  A genome-scale library of human ORFS in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.

Non-Mammalian cDNAs and ORFs

Your complete source for cDNAs, ORFs, knockout strains, promoter collections and other resources for yeast, C. elegans, Zebrafish, Xenopus, and E. coli.

• Yeast

  Browse our list of yeast resources including Yeast Knockout strains, Tagged-ORF strains, Genomic DNA, cDNA libraries, and additional collections.

• C. elegans

  C. elegans ORFs, RNAi feeding collections, promoter and transcription factor collections.

• E. coli

  Includes the E. coli Keio knockout collection, Promoter-GFP fusions for monitoring gene expression, and tagged ORFs for studying protein-protein interactions.

• Zebrafish

  Full-length, fully-sequenced Zebrafish ZGC and IMAGE clones, as well as Exelixis and NEIBank cDNAs and the Zebrafish IMCB Collection.

• Xenopus

  Full-length, fully-sequenced Xenopus ORFeome, XGC, and IMAGE cDNA and EST clones.

cDNA and ORF Libraries

• Cherry-Pick Library Tool

  Upload your gene list to design and order a custom cDNA or ORF library.

Pre-defined gene family collections are available for many of our cDNA and ORF products. Choose from whole genome, gene families, or custom libraries.

• Mammalian Gene Collection Libraries

  The mouse and human MGC libraries contain popular gene families in expression-ready and other vector backbones.

• CCSB Human ORFeome Libraries

  

  The Gateway adapted human Entry ORF libraries are ideal for easily moving entire families of ORFs into your choice of protein expression systems.

• Precision LentiORFs

  Precision LentiORFs are expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.

• ORFeome Collaboration Libraries

  

  ORFeome Collaboration clones are sequence-verified, human open reading frames (ORFs) subcloned into Gateway recombinational entry vectors.

• CCSB-Broad Lentiviral Expression Library

  

  A genome-scale library of human ORFS in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.

• Human ORFeome V8.1 Library

  This fully-sequenced collection of Gateway-adapted ORFs represents the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.

• Non-mammalian cDNA & ORFs
  

  Find cDNA and ORF libraries for E. coli, C. elegans, X. laevis, D. rerio, and yeast genes.

Horizon (formerly Dharmacon) is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Horizon and thus the quality of the collections is largely dependent upon what Horizon received from these groups. Specific clone information and plate coordinates were provided to Horizon by the suppliers of these clone collections. Horizon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Horizon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Horizon , the clone you receive might not match the expected clone. If this occurs, please contact Technical Support.

All clones and plates are provided as glycerol stocks. Individual strains are shipped at room temperature. Plates are shipped on dry ice. Both should be stored at -80ºC.