Cas9 nuclease solutions for every CRISPR gene editing workflow
Horizon offers a variety of Cas9 nuclease solutions for CRISPR-based gene editing experiments.
Learn more about the Edit-R™ CRISPR-Cas9 system and gene editing applications.
Cas9-expressing stable cell lines for streamlined gene editing
Shorten your next gene editing workflow with "CRISPR-ready" Cas9-expressing stable cells in a variety of popular cell types.
Fluorescent Cas9 reagents for optimization and enrichment
Transient Cas9 products for DNA-free gene knockout
Lentiviral Cas9 for easy delivery into any cell type
Eliminate the need for cytotoxic transfection or electroporation steps with constitutive or inducible lentiviral Cas9 reagents.
Cas9 nuclease selection guide
Determining the most appropriate Cas9 nuclease reagent for your experiment is dependent on the particular application or cell type.
See the table below to determine the best format for your experiment.
|Cas9 protein||Cas9 mRNA||Lentiviral Cas9 particles|
|DNA-free, transient expression||✔||✔|
|Co-electroporate with synthetic guide RNA||✔||✔|
|Co-transfect with synthetic guide RNA||✔||✔|
|Enrich population with FACS||✔||✔|
|Enrich population with resistance marker||✔|
|Create stable cell lines||✔|
|Lentiviral transduction for cells that are difficult to transfect||✔|
Cas9 nuclease sources
"CRISPR-ready" premade Cas9-expressing cell lines
Transient Cas9 nuclease for DNA-free workflows
Vector-based solutions for generating Cas9-expressing cell lines
CRISPR guide RNA
Edit-R synthetic guide RNA & controls
Genome wide synthetic sgRNA designs guaranteed to edit your gene of interest. The design algorithm maximizes the potential for functional protein knockout while minimizing off-target editing through stringent specificity checks.
Species-specific synthetic sgRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.
Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific sgRNA.
Guaranteed to edit your target! Algorithm-optimized crRNA for genome-wide coverage of human and mouse genes. Modifications for nuclease resistance improve DNA-free editing.
Trans-activating CRISPR RNA (tracrRNA) is required for use with Edit-R synthetic crRNA to form the complex that programs Cas9 nuclease.
Edit-R lentiviral sgRNA & controls
Combined Cas9 and sgRNA expression for efficient gene knockout & unparalleled specificity; available as glycerol stocks and high-titer purified particles.
All-in-one lentiviral sgRNA controls to verify DNA double-strand breaks and gene editing efficiencies.
All-in-one lentiviral sgRNA constructs bioinformatically designed to not target any gene in human or mouse genomes.
Guaranteed to edit your target, algorithm-optimized sgRNA for genome-wide coverage of human or mouse genes. Provided as high-titer lentiviral particles or glycerol stocks.
Species-specific sgRNAs targeting well-characterized genes to determine the effectiveness of your gene editing conditions for maximum efficiency.
Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene-specific sgRNA.
Custom guide RNA design
Place a custom guide RNA order, or design and order your own synthetic sgRNA, crRNA, or lentiviral sgRNA with our easy-to-use interface.
Edit-R HDR donor template design, ordering tools & kits
Design and order a single-strand DNA donor (≤ 150 nt) for insertion, deletion, or other alteration.
Design and order a plasmid DNA donor kit for insertion of a mKate2 or EGFP fluorescent marker, or other a custom insert.
Rapidly and easily assemble a plasmid donor for HDR
Quickly and efficiently build a HDR donor plasmid
PCR components for Edit-R Plasmid Donor Kits
The Edit-R guarantee
We guarantee that EVERY predesigned guide RNA will provide successful editing at the target site when delivered as described in the Edit-R technical manuals.
The Edit-R guide RNA guarantee is valid when used with any wild type S. pyogenes Cas9 nuclease, including mRNA, expression plasmid, protein, or stable Cas9 expression, and Edit-R crRNAs must be used with Edit-R tracrRNA for the guarantee to apply.
Analysis of editing of the treated cell population must be shown using a T7EI or Surveyor mismatch detection assay. If successful editing is not observed for a predesigned Edit-R guide RNA while an appropriate side-by-side Edit-R positive control is successful, a one-time replacement of a different predesigned Edit-R guide RNA of the same format and quantity will be provided at no cost.
A replacement will only be approved upon discussion with our Technical Support team.
Successful editing at the DNA level does not always lead to functional gene knockout; it is recommended to test multiple guide RNAs to determine the most effective guide RNA for knockout of your target gene.
This guarantee does not extend to any accompanying experimental costs, does not apply to guide RNAs ordered via the CRISPR Design Tool, and will not be extended to the replacement guide RNA.