当社のプレミアムなshRNA製品は、microRNAベースshRNAデザインを採用しており、細胞内でより効率的にプロセッシングされ、RNAi実験における毒性が低減する可能性があることが示唆されています。詳細については、shRNAアプリケーションのページをご覧ください。
しかしながら、効率的な遺伝子ノックダウンは、shRNAデザインと発現レベルの両方に依存します。したがって、特定の細胞株で十分なshRNA発現を促進するためには、プロモーターの選択も重要です。
SMARTvector™ – 実験に合わせた柔軟な製品選択が可能なshRNA
SMARTvectorバックボーンでは、7つのプロモーターと3つのレポーターオプションから選択し、特定の細胞や実験要件に合わせてshRNAを最適化できます。
最適化されたプロモーターの下で発現されるSMARTvectorレンチウイルスshRNAは、最先端のmicroRNAベースのshRNA固有の合理的デザインアルゴリズムを使用してデザインされており、非常に強力で特異的なノックダウンを実現します。SMARTchoice promoter selection plateを使用して、細胞株に最適なプロモーターを特定することができます。
SMARTvector shRNAは、Tet-on®3G誘導性プロモーターとともに利用可能で、可逆的で制御された遺伝子ノックダウンを実現します。これは、必須遺伝子の機能を研究する場合に特に役立ちます。
GIPZ/TRIPZコレクション
GIPZ shRNAおよびTRIPZ誘導性shRNAは、細胞毒性を最小限に抑えた特異的な遺伝子ノックダウンを提供しますが、SMARTvectorで実現できるようなプロモーター／レポーターの選択柔軟性は不足しています。GIPZコレクションとTRIPZコレクションはどちらも、Gregory Hannon（Cold Spring Harbor Laboratories）とSteve Elledge（Harvard）により共同開発されたものです。
Order shRNA reagents
SMARTvector lentiviral shRNA
Guaranteed gene silencing with multiple promoter and reporter options.
SMARTvector inducible lentiviral shRNA
Smartvector lentiviral shRNA with Tet-inducible technology for unprecedented silencing control.
GIPZ lentiviral shRNA
Efficient gene silencing with a microRNA-adapted shRNA design.
TRIPZ inducible lentiviral shRNA
Inducible shRNA expression for tightly regulatable RNAi experiments.
TRC lentiviral shRNA
Lentiviral shRNA collection from The RNAi Consortium (TRC) for human and mouse cells.
製品選択ガイド
表をご参照の上、実験目的に適したshRNA試薬をご選択ください。
| SMARTvector shRNA | GIPZ | TRIPZ | TRC | |
|---|---|---|---|---|
| 生物種 | Human, Mouse, Rat | Human, Mouse | Human | Human, Mouse |
| プロモーター |
恒常発現：7種類より* 誘導発現：4種類より |
Human CMV Pol II | TRE-min-CMV | U6 Pol III |
| Vector backbone | Lentiviral | Lentiviral | Lentiviral | Lentiviral |
| ステムループ形式 | microRNAベース | MimicroRNAベース | microRNAベース | シンプルヘアピン型 |
| 蛍光レポーター遺伝子 | GFP/RFP | GFP | RFP | |
| ノックダウン保証** | ||||
| In vivo RNAi | ||||
| 安定した細胞株の作成 | ||||
| 初代細胞や非分裂細胞に推奨 | ||||
| 誘導発現用 | ||||
| 全ゲノムライブラリー | Human, Mouse, Rat | Human, Mouse | Human | Human, Mouse |
| 製品形態 |
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*一部のプロモーターオプションは、カスタム製品として、またはリクエストに応じてのみ利用できる場合があります。
**SMARTvector、GIPZ、およびTRIPZレンチウイルスshRNAの場合、3つのコンストラクトのうち少なくとも1つは、適切なnon-targetingおよびポジティブコントロールと組み合わせて使用した場合、ターゲットmRNAレベルが70％以上減少することが保証されます。保証の詳細は各製品ページをご覧ください。
SMARTvector shRNA reagents & controls
Critical to any gene silencing experiment, shRNA controls enable accurate interpretation for reliable, reproducible results.
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SMARTvector lentiviral shRNA
Guaranteed gene silencing with multiple promoter and reporter options.
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SMARTvector lentiviral controls
Positive and negative constitutive shRNA controls with choice of seven promoters and three reporter options.
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SMARTvector inducible lentiviral shRNA
Smartvector lentiviral shRNA with Tet-inducible technology for unprecedented silencing control.
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SMARTvector inducible lentiviral controls
Inducible expression of positive and negative controls with your choice of four promoters and two reporters.
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SMARTchoice promoter selection plate
Identify the optimal SMARTvector promoter option for your cell line.
shRNA collections & controls
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GIPZ lentiviral shRNA
Efficient gene silencing with a microRNA-adapted shRNA design.
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GIPZ lentiviral controls
Validated collection of GIPZ positive and negative controls for a well-designed RNAi experiment.
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TRIPZ inducible lentiviral shRNA
Inducible shRNA expression for tightly regulatable RNAi experiments.
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TRIPZ inducible lentiviral controls
Positive and negative TRIPZ Inducible Lentiviral shRNA Controls for setting the experimental window.
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TRC lentiviral shRNA
Lentiviral shRNA collection from The RNAi Consortium (TRC) for human and mouse cells.
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TRC lentiviral controls
Positive shRNA control targeting GFP and empty lentiviral vector negative control available as glycerol stocks
Pooled shRNA screening libraries
A pooled lentiviral screen can be performed to identify genes that regulate cellular responses and signaling pathways, or to discover novel gene functions. Pooled screening libraries can consist of as few as 50 constructs up to many thousands. In contrast to the costly automated techniques that are required to screen using individually arrayed reagents, pooled screening libraries allow the researcher to transduce and screen a population of cells within a few tissue culture dishes.
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Custom pooled lentiviral screening libraries
Flexible collections of lentiviral shRNA for customizable RNAi knockdown screens.
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SMARTvector lentiviral shRNA pooled libraries
High-titer pooled screening libraries of constitutive SMARTvector Lentiviral shRNAs for pre-defined gene libraries in human, mouse, and rat. Choose from seven promoters and three reporter options to select the best format for your experiment.
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SMARTvector inducible lentiviral shRNA pooled libraries
High-titer pooled screening libraries of SMARTvector Inducible Lentiviral shRNAs for pre-defined gene libraries in human, mouse, and rat. Choose from four promoters and two reporter options to select the best format for your experiment.
Arrayed shRNA screening libraries
Delivered as glycerol stocks in 96-well format; these libraries are ideal for arrayed screening formats to elucidate gene activity and study specific pathways or gene families.
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Custom cherry-pick clone libraries
Upload your gene list to build your own custom arrayed shRNA library.
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GIPZ lentiviral shRNA libraries
Genome-wide collections for high-throughput shRNA screening
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TRIPZ inducible lentiviral shRNA libraries
Genome-scale TRIPZ Inducible Lentiviral shRNA library arrayed in 96-well plates
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TRC lentiviral shRNA libraries
Simple hairpin shRNAs in the pLKO.1 lentiviral vector designed by The RNAi Consortium (TRC)
The SMARTvector functional guarantee
When you purchase a minimum of three SMARTvector lentiviral shRNAs to the same protein-coding gene target using the optimal SMARTchoice promoter for your cell type, at least one of the shRNA constructs will reduce target mRNA levels by 70% or more when used with the vector matched non-targeting control and GAPD or PPIB positive control. Optimal promoters should be determined with the SMARTchoice promoter selection plate. mRNA levels should be measured with RT-qPCR or similar quantitative mRNA analysis no earlier than 72 hours post-transduction.
The guarantee only applies to catalog numbers V3S*xxxx and does not apply to any SMARTvector shRNAs targeting lncRNA transcripts.
GIPZ/TRIPZ functional guarantee
When you purchase a minimum of three shRNA to the same target, at least one of the shRNA constructs will reduce target mRNA levels by 70% or more when used with Starter Kit protocols and normalized for delivery efficiency. Delivery conditions should be confirmed using appropriate positive controls provided in the kit and the percent-knockdown should be compared to cells using the negative control (non-targeting) shRNA.
Please note that the GIPZ Lentiviral shRNA and TRIPZ Inducible Lentiviral shRNA are not compatible with third generation packaging systems, such as ViraPower from Invitrogen. We recommend the Trans-Lentiviral Packaging System to general lentiviral particles for transduction of GIPZ and TRIPZ shRNA.