HDR Donor Designer Workflow

Quickly and easily design and order a donor template for precise CRISPR-Cas9 gene editing!

Rapidly design and order custom donor plasmids for insertion of fluorescent markers or custom inserts, or generate custom-synthesized ssDNA donor oligonucleotide (oligo) templates for precise insertion, removal, or replacement of genomic DNA.

  • Leverage the power of CRISPR-Cas9 genome engineering to make a precise change in a target gene sequence for human, mouse, or rat
  • Donor oligo modified with two phosphorothioate bonds on the 5’ and 3’ ends to provide a > 2-fold improvement in knock-in efficiency (vs non-modified oligo
  • Donor oligo length of up to 150 bases allows for sequence insertion, removal, or replacement, and symmetric or asymmetric donor oligo configuration
  • Design a plasmid donor for insertion of an mKate2 or EGFP fluorescent marker or a fully custom insert at any desired genomic site
  • Intuitive interface lets you accurately design and visualize changes to the gene as well as the finished DNA template

Following a double-strand break (DSB) by CRISPR-Cas9, homology-directed repair (HDR), is a repair pathway that requires a donor template with sequence homology adjacent to the desired genomic modification site. A well-designed donor template can introduce precise alterations to the genomic sequence to facilitate nucleotide insertion, removal or replacement. The Edit-R HDR Donor Designer facilitates straightforward donor template design for your gene of interest.

Here’s How

Edit-R HDR Donor Designer Workflow

First, use our online CRISPR Design Tool to design and order the CRISPR-Cas9 guide RNA required to cut the genomic DNA in close proximity to the desired modification site. Next, use the Edit-R HDR Donor Designer for rapid generation of the donor template to complete your HDR experiment:

  1. Input gene identifiers for your desired target
    • Organism
    • NCBI gene ID or Gene symbol
  2. Enter the target sequence for the gRNA that will be used to cut the genomic DNA
  3. Specify the desired genomic site to be altered
    • Oligo donors: Designate a sequence to insert, remove or replace
    • Plasmid donors: Indicate the site of mKate2, EGFP, or custom sequence insertion
  4. Configure your desired DNA template(s), add to your cart and check out!