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- Mimix™ Quantitative Multiplex (FFPE) Reference Standard
Mimix™ Quantitative Multiplex (FFPE) Reference Standard
The Quantitative Multiplex FFPE Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations.
Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The Quantitative Multiplex Reference Standard (QMRS) portfolio covers multiple endogenous SNPs, insertions and deletions. The QMRS includes 11 mutations at 0.8-24.5% allelic frequency in genomic DNA, FFPE and Formalin-Compromised DNA format. The multiple formats of the QMRS allow you to validate both the pre-analytical and analytical portion of your workflow using an isogenic reference standard. The FFPE format QMRS allows you to evaluate and optimize DNA extraction protocols without wasting precious patient samples. With the Formalin-Compromised format, the robustness of your NGS workflow may be tested to ensure that your library protocol and informatics pipeline can accommodate these highly degraded samples. The QMRS standard is our most popular multiplex standard as it provides both a breadth of formats, but also a large range of allelic frequencies and onco-relevant mutants.
With this product you are able to:
- Evaluation of workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics
- Analyze the robustness of your workflow with multiple formats (DNA , FFPE and Formalin-Compromised DNA)
- Optimize and validate new cancer panels and routinely monitor the performance of your assay
Product Information
Product Description: Quantitative Multiplex Reference Standard
Format: FFPE
Genes Covered: BRAF, KIT, EGFR, KRAS, NRAS, PIK3CA
Fixation Method: 4% PFA (w/v)
Section Size: 15µm
Cell Density: 3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA Yield: > 400 ng DNA using Promega Maxwell RSC Plus Extraction kit
Verified Mutations:
Chromosome |
Gene |
Variant |
Expected Allelic Frequency, % |
7q34 |
BRAF |
V600E |
10.70% |
4q11-q12 |
cKIT |
D816V |
10.00% |
7p12 |
EGFR |
ΔE746 - A750 |
1.90% |
7p12 |
EGFR |
L858R |
2.80% |
7p12 |
EGFR |
T790M |
0.90% |
7p12 |
EGFR |
G719S |
24.50% |
12p12.1 |
KRAS |
G13D |
15.00% |
12p12.1 |
KRAS |
G12D |
6.30% |
1p13.2 |
NRAS |
Q61K |
12.50% |
3q26.3 |
PIK3CA |
H1047R |
17.50% |
3q26.3 |
PIK3CA |
E545K |
8.80% |
Presence confirmed in parental cell line
Chromosome |
Gene |
Variant |
Expected Allelic Frequency, % |
2p23 |
ALK |
P1543S |
33% |
1q25.2 |
ABL2 |
P986fs |
8% |
5q21-q22 |
APC |
R2714C |
33% |
1p35.3 |
ARID1A |
p.M1564fs*1 |
33.50% |
13q12.3 |
BRCA2 |
K1691fs |
33% |
13q12.3 |
CDX2 |
V306fs |
41.50% |
22q13.2 |
EP300 |
K292fs |
8% |
4q31.3 |
FBXW7 |
S668fs |
33.50% |
8p12 |
FGFR1 |
P150L |
8.50% |
13q12 |
FLT3 |
V197A |
11.50% |
2q33.3 |
IDH1 |
S261L |
10% |
7q31 |
MET |
L238fs |
6.50% |
3p21.3 |
MLH1 |
L323M |
8.50% |
17q11.2 |
NF1 |
Y628fs |
7.50% |
22q12.2 |
NF2 |
P275fs |
8% |
9q34.3 |
NOTCH1 |
P668S |
31.50% |
1q21-q22 |
NTRK1 |
5'UTR (1:156815754, GRCh38) |
8.50% |
4q12 |
PDGFRA |
G426D |
33.50% |
Unit Size: 1 FFPE Section
Extractable DNA: ≥ 400 ng DNA per section using Promega Maxwell RSC Plus FFPE DNA Extraction kit
Intended Use: Research Use Only
Exome sequencing data: Download additional information on all exonic variants present in this product. Please note: this NGS data is not batch specific.
General Information
Allelic Frequency: 1% - 24.5%
Storage: 4˚C
Shipping: Ambient
Expiration: See all product shelf life information
Quality Control
Genotype and Allelic Frequency: Droplet Digital PCR™
Quality: Agarose gel electrophoresis
Quantification: Quantifluor™
Manufacturing Quality: ISO 13485:2016
We accept orders using the following methods.
Online
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
Offline
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
- Shipping address and billing address with contact names
- Purchase Order number if you have one
- Telephone number
- VAT Number (European Countries only)
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