An RNAi transfection control for human, mouse, or rat that allows high-throughput assessment of transfection efficiency based on cell death. This control is quick and easy to use, requiring only visual inspection of cells.
TOX Transfection Control provides a simple method for assessing transfection efficiency and is cytotoxic only when productively delivered into cells. Cells successfully transfected with the TOX Transfection Control typically undergo apoptosis and cell death within 24 to 48 hours.
This control is partcularly suited for high-throughput siRNA screening when a rapid visual inspection is useful to qualitatively confirm that transfection was successful.
Highlights
Novel control for qualitative assessment of transfection success in RNAi experiments
Successful transfection leads to apoptosis and cell death in 24 to 48 hours
Requires only visual inspection of cells - no downstream assays required
Experimental considerations
The TOX Transfection control, like other cell death controls, has demonstrated variation in the degree of cell death across different cell types. The efficacy of the control likely requires intact cellular mechanisms, including the apoptosis and interferon pathways. As with any RNAi control, assessment of efficacy in your particular cell type and assay conditions is recommended prior to larger scale use.
Concentrated buffer solution recommended for resuspension and long-term storage of any short, double-strand, or single-strand synthetic RNA molecule. Dilute with RNase-free water prior to use.
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments
