Dharmacon™ Edit-R™ has been featured in over 20 peer-reviewed publications since 2015
Check out our recommended reading list! It includes the Dharmacon Edit-R CRISPR-Cas9 system, and custom crRNAs and RNAs, used for gene editing in mammalian cells and in other exciting, creative applications.
Did you know that synthetic crRNA:tracrRNA with Cas9 protein can be delivered as a ribonucleoprotein for functional genetic screening in primary human T cells (Hultquist, 2016)?
Or, for instance, that CRISPR-Cas9 with synthetic RNAs can be used for identification of plasmids that confer bacterial resistance (Müller, 2016)?
Edit-R has also been successfully applied to uncover gene functions in nematodes (Delaney, 2017 and Paix, 2015).
Publications with Dharmacon Edit-R CRISPR-Cas9 reagents
- C. E. Delaney, A. T. Chen, et al. A histone h6 lysine 20 methyltransferase couples environmental cues to sensory neuron control of developmental plasticity. Development. 144, 1273-1282 (2017).
- N. Maio, K. S. Kim, et al. A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III. Cell Metab. 25, 945-953, e6 (2017).
- L. J. Rupp, K. Schumann, et al. CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells. Sci Rep. 7, 737 (2017).
- Ž. Strezoska, M. Perkett, et al. High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries. J. Biotechnol. 251, 189-200 (2017).
- X. M. van Wijk, S. Döhrmann, et al. Whole-Genome Sequencing of Invasion-Resistant Cells Identifies Laminin α2 as a Host Factor for Bacterial Invasion. MBio. 8, e02128-16 (2017).
- T. A. Aguilera, M. Rafat, et al.. Reprogramming the immunological microenvironment through radiation and targeting Axl. Nat Commun. 7, 13898 (2016).
- K. He, E. Chou, et al. Conjugation and evaluation of triazole-linked single guide RNA for CRISPR-Cas9 gene editing. ChemBioChem. 17, 1809–1812 (2016).
- R. Eggenschwiler, M. Moslem, et al. Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase. Sci Rep. 6, 38198 (2016).
- J. F. Hultquist, K. Schumann, et al. A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells. Cell Reports 17, 1438–1452 (2016).
- M. L. Kelley, Ž. Strezoska, et al. Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing. J. Biotechnol. 233, 74–83 (2016).
- J. McCaffrey, J. Sibert, et al. CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double strand DNA for whole genome mapping and structural variation analysis. Nucleic Acids Res. 44, e11 (2016).
- V. Müller, F. Rajer, et al. Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping. Sci Rep. 6, 37938 (2016).
- A. Paix, H. Schmidt, et al. Cas9-assisted recombineering in C. elegans: genome editing using in vivo assembly of linear DNAs. Nucleic Acids Res. 44, e128 (2016).
- J. Tan, S. E. Martin. Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening. PLOS ONE 11, e0168968 (2016).
- E. M. Anderson, A. Haupt, et al. Systematic analysis of CRISPR-Cas9 mismatch tolerance reveals low levels of off-target activity. J. Biotechnol. 211, 56-65 (2015).
- R. Barrangou, A. Birmingham, et al. Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference. Nucleic Acids Res. 43, 3407–3419 (2015).
- H. Ogiwara, M. Sasaki, et al. Targeting p300 addiction in CBP-deficient cancers causes synthetic lethality by apoptotic cell death due to abrogation of MYC expression. Cancer Discov. 6, 430-445 (2015).
- S. Opp, D. A. S. A. Vieira, et al. MxB Is Not Responsible for the Blocking of HIV-1 Infection Observed in Alpha Interferon-Treated Cells. J. Virol. 90, 3056-3064 (2015).
- A. Paix, A. Folkmann, et al. High efficiency, homology-directed genome editing in Caenorhabditis elegans using CRISPR-Cas9 Ribonucleoprotein complexes. Genetics 201, 47-54 (2015)
- G. Sivan, P. Ormanoglu, et al. Identification of Restriction Factors by Human Genome-Wide RNA Interference Screening of Viral Host Range Mutants Exemplified by Discovery of SAMD9 and WDR6 as Inhibitors of the Vaccinia Virus K1L-C7L- Mutant. MBio. 6, e01122 (2015).
- W. Deng, X. Shi, et al. CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc Natl Acad Sci U S A. 112, 11870–11875 (2015).
Find out about all of these applications on our CRISPR-Cas9 Recommended Reading List »
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