Every year since 2007, The Scientist Magazine gets hundreds (thousands?) of nominations for their Top 10 Innovations Award.
Their goal is to recognize cutting-edge technologies that have the potential to dramatically change, and improve, life science research. Over the years, this recognition has spanned instruments to reagent kits and everything in between.
This year’s winners
One of the 10 awards this year went to Edit-R™ Fluorescent Cas9 nuclease mRNA – a DNA-free source of Cas9 for CRISPR gene editing that co-expresses either mKate2 or EGFP. This enables researchers to perform genome engineering experiments in a completely DNA-free manner while still retaining the ability to enrich for transfected, and therefore edited, cells in a population.
Four key benefits to using fluorescent Cas9 mRNA
- Fluorescent Cas9 mRNA enables enrichment of an edited cell population, either knockout or knock-in, without exogenous DNA or lentivirus.
- A DNA-free system (Cas9 mRNA and synthetic guide RNA) eliminates the possibility of incorporating unwanted, exogenous DNA into the host cell line genome
- Compatible with transfection or electroporation delivery approaches
- Transient expression of Cas9 nuclease may reduce off-targeting
A history of innovation
Horizon Discovery and Dharmacon products have been fortunate to be recognized for our ongoing dedication to continued innovations and improvements for the research community.
Year |
The Scientist Top 10 Award Winner |
|---|---|
|
2015 |
On-demand deletions in human HAP1 cells |
|
2016 |
Horizon Turbo GFP-tagged HAP1 cells |
|
2017 |
Edit-R crRNA arrayed Library—Human Genome |
|
2018 |
Edit-R Fluorescent Cas9 nuclease mRNA |
Additional Resources
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Fluorescent Cas9 mRNA for enrichment of CRISPR-mediated knockout and knock-in using synthetic guide RNA - Application Note
A demonstration of the use of Edit-R Fluorescent Cas9 Nuclease mRNA and synthetic guide RNAs for enriching knockout and knock-in gene edited cells. -
Edit-R Cas9 Nuclease mRNA
Purified Cas9 Nuclease mRNA for co-transfection with synthetic guide RNA for a completely DNA-free genome engineering system -
Edit-R Cas9 mRNA, synthetic guide RNA, and HDR donor template transfection - Quick Protocol
A quick protocol for transfecting Edit-R Cas9 mRNA, synthetic guide RNA, and HDR donor template into mammalian cells using DharmaFECT Duo