RNAiを成功させるためのヒントとコツ：トランスフェクション時に必要なコントロールを含めてください！
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After all the hard work of editing your cell line, you want to have confidence in your new research model. So, how do you verify your cell line is what you expect it to be? Could a heterogeneous cell population be obscuring your editing effects? Is observed phenotype being caused by the targeted gene edit, or unintended off-target effects? Here we discus ways to add supporting data to validate your gene-engineering projects.
The only self-delivering siRNA for difficult-to-transfect cells carries a functional guarantee.
CRISPRテクノロジーは、遺伝子と分子パスウェイをより明確に調べることを可能にしました。どのように、ノックアウト細胞株を、遺伝子レスキューと疾患変異モデルとともに、または独立した細胞モデルとして使用して、研究を検証し、研究成果を拡張できるかを説明しています。
Top ten recommendations to validate your NGS assay.
Essential genes are defined as genes that are critical for the survival of an organism. These are considered to be genes that are absolutely required for the cell to grown, proliferate and survive. Deletion of an essential gene from a cell eventually leads to the death of this cell or a severe proliferation defect. As a consequence, it is impossible to generate cells with a knock-out or deletion of essential genes.
Nuclease resistance improves performance of synthetic CRISPR RNA with Cas9 mRNA
Following transfection of CRISPR reagents, cells will need to be single cell diluted to obtain a clonal population. In this blog, we review the method used in-house.
A review of the main 5 tips to choose and use a cell line. We highlight the importance of checking for authenticity, and contamination.