Revvity molecular reference standards are available for both germline and somatic variants. In this article we cover why this distinction is important, and what it means for the allele frequencies of variants in these two types of controls.
Functional genomic screening allows for the elucidation of the mechanism of action of exogenous agents which modulate intracellular gene function such as pharmaceutical agents and other compounds. Read about the various considerations, approaches and interpretations when conducting a screen to investigate novel compounds and analyzing the screening data.
With the requirement of thorough in vitro assays and high throughput screening technologies to ensure drug safety and efficacy, make sure you're fully ready for when you submit your IND application by assessing immunogenicity with an MLR assay
プール化CRISPR薬剤遺伝子スクリーニングでは、ポジティブまたは耐性スクリーニングとネガティブまたは感受性スクリーニングという2つの選択メカニズムを適用することができ、それぞれに様々な考慮事項や使用法があります。
Functional Genomic and Cell Panel Screening can individually provide comprehensive data sets to support therapeutic development, but when utilized in combination they identify novel targets which are cross-validated by independent approaches.
CRISPRを用いてノックアウト細胞株を作製する際、ウエスタンブロッティングは細胞株の検証に役立つ有用なツールである。しかし、結果を正しく解釈することは極めて重要である。ここでは、ノックアウト後に起こりうるタンパク質の運命をまとめ、ノックアウト細胞株を検証する最善の方法を探る。
Learn about how T cell exhaustion poses a significant challenge in immuno-oncology and the way epigenetic mechanisms can help develop strategies for reinvigorating exhausted T cells.
間葉系から上皮系への移行（MET）の維持に関与する遺伝子を対象に、3つのLOF技術（CRISPRko、CRISPRi、siRNA）による直交標的検証戦略を採用した研究を紹介します。
Given the enthusiasm for adopting CRISPR-based approaches, let’s take a look at the known limiting factors in using arrayed lentiviral resources, and what we can learn from this work as CRISPR arrayed screening advances as a discovery method.
機能的および特異的なガイドRNAのためのDharmacon™ Edit-R™ CRISPRノックアウトアルゴリズムの開発おける考慮事項についてご説明していています。