Here we talk about the role of the Edit-R Pooled sgRNA Indexing PCR Primers and answer the most common questions about the Indexing PCR and Sequencing Primer kit.
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Achieve DNA-free guaranteed gene editing with synthetic predesigned sgRNA reagents and libraries covering the entire human genome.
Did your initial round of RNAi screening produce some hits? How do you determine which hits are true positives?
Check out the BioIT Beta Tools page to test tools created by our BioIT team!
Sometimes a human promoter is optimal in mouse cells and vice versa. Dharmacon has solutions to investigate the best promoter for your cell type.
利用できる多くの選択肢の中から最適なセルベーススクリーニングを選ぶことはたちまちに意思決定が複雑になることがあります。ここでは、主な選択肢である細胞パネルスクリーニングと機能ゲノミクススクリーニングを概観します。
Functional genomic screening libraries that represent the most current findings within the field
CRISPR-Cas9 lentiviral sgRNA pooled screening changes everything.
The clinical success rate of new oncology drugs is only 3.4% compared to 20.9% in other disease types (Wong et al, 2018). One contributing factor to this issue is the testing systems used, with two-dimensional (2D) monolayer assay formats as the traditional mainstay of high throughput screening. Although 2D monolayer assays have identified many successful drugs, it is increasingly recognized that they do not accurately model key aspects of the three-dimensional (3D) tumor environment. Therefore, the adoption of high throughput screening approaches using 3D assays to complement 2D approaches could substantially improve prediction of clinical outcomes and reduce the high failure rate of cancer drugs in clinical trials.
A major study has been undertaken to gain a better understanding of thousands of mutations in the BRCA1 gene - a key gene in breast and ovarian cancers.