Revealing the role of E3 ubiquitin ligases in DNA damage repair. One of the diverse new uses for the HAP1 cell line, one that has begun to draw significant attention, is in the field of DNA damage repair. A recent paper from Minoru Takata's group highlights this important application of this relatively new tool.
Essential genes are defined as genes that are critical for the survival of an organism. These are considered to be genes that are absolutely required for the cell to grown, proliferate and survive. Deletion of an essential gene from a cell eventually leads to the death of this cell or a severe proliferation defect. As a consequence, it is impossible to generate cells with a knock-out or deletion of essential genes.
Cancer fusion genes are hybrid genes that produce abnormal proteins believed to catalyze further cancer growth and increase invasiveness. Here we highlight novel research using CRISPR gene editing to specifically target cancer fusion genes, which could offer many potential therapeutic applications.
A look at how a new method dubbed SHERLOCK adapts CRISPR-based technology to a diagnostic platform to allow mutation detections in DNA and RNA.
This blog explores how a selection of HAP1 knockout cell lines were used to confirm the involvement of DDR pathways in compounds mode of action, demonstrating opportunities for the exploitation of synthetic lethal interactions.
We explore the different ways to visualize proteins and check their expression. We will highlight in this blog the limits and drawbacks to their use.
Nuclease resistance improves performance of synthetic CRISPR RNA with Cas9 mRNA
Review of a paper published in Nature .com, detailing analysis of CRISPR-Cas9 sensitivity (Drop-out) screening, which resulted in the development of a streamlined screening pipeline to evaluate fundamental aspects of functional genomic screening.
The scientists at Horizon Discovery have published a robust and precise approach to generating translocations. This advancement facilitates the generation of relevant cell line models for oncology research.
Next-generation sequencing (NGS), has become a universal tool in diverse industries; most recently moving into the clinic for patient diagnosis. However, if labs wish to analyze patient-derived materials they must first face a hurdles labs to optimize and validate their workflow including determining the exact test limitations.