Mimix™ Prostate Cancer Panel, cfDNA Reference Standard
Prostate Cancer Panel cfDNA Reference Standardは、ISO: 13485に基づいて製造された、特性のよく明らかにされている細胞株由来の精度管理用コントロールであり、前立腺がんに関連する9遺伝子の15のバリアントを含みます。検査室では、この新しいMultigene Multiplex製品により、前立腺がんの配列解析において迅速で信頼性が高く費用対効果の高いバリデーションを実行できます。このことは、品質保証の目標をより迅速に達成するための陽性コントロールとなる患者試料の入手の不確実性を低減し、時間とコストを節約し、cfDNAアッセイの性能を評価することができると考えられます。
Technical data
フォーマット：cfDNA
対象遺伝子：TP53, AR, PIK3CA, RB1, CDKN2A, STK11, PTEN, BRCA1, BRCA2
アレル頻度：0.3% - 10%
Buffer：Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Product information
フラグメント長：160 bp
ユニットサイズ：350 ng/vial
濃度：20 ng/µl
検証済み変異：
| Gene | DNA sequence change | Amino acid change | Expected allelic frequency (%) |
|---|---|---|---|
| TP53 | c.992A>G | p.Q331R | 0.60 |
| TP53 | c.742C>T | p.R248W | 9.00 |
| TP53 | c.668C>T | p.P223L | 0.50 |
| TP53 | c.820G>T | p.V274F | 1.00 |
| TP53 | c.414_del | p.A138fs*31 | 10.00 |
| AR | c.2623C>T | p.H875Y | 0.30 |
| AR | c.2623A>G | p.T878A | 0.30 |
| PIK3CA | c.1637A>G | p.Q546R | 0.70 |
| PIK3CA | c.1624G>A | p.E542K | 1.20 |
| RB1 | c.2143A>T | p.K715* | 1.00 |
| CDKN2A | c.250G>T | p.D84Y | 0.60 |
| STK11 | c.532_536del | p.KP178Gfs*86 | 1.20 |
| PTEN | c.17_18del | p.K6Rfs*4 | 0.50 |
| BRCA1 | c.532dup c.5263_5264insC |
p.Q1777Pfs*74 p.Q1756Pfs*74 |
0.40 |
| BRCA2 | c.9924C>G | p.Y3308* | 0.40 |
General information
保管温度：4℃
有効期限：product shelf life informationを参照してください。
Quality control
断片化サイズ：D1000 ScreenTape アッセイ
アレル頻度：Droplet Digital™ PCR
定量化：Qubit® dsDNA BRアッセイ（Invitrogen）
We accept orders using the following methods.
Online
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
Offline
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
- Shipping address and billing address with contact names
- Purchase Order number if you have one
- Telephone number
- VAT Number (European Countries only)
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Selection guides
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Special Discount Pricing: The Prostate Cancer Panel cfDNA Reference Standard (Negative Control) is a well characterized, cell line-derived control material manufactured under ISO:13485 that is negative for 15 variants across 9 genes clinically relevant to prostate cancer.