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How was the batch-specific supplementary NGS data produced and analysed?
The following reference standards are offered with batch-specific NGS data: HD848, HD846, HD867, HD852, HD876, HD852-IVD, HD876-IVD.
HD848 and HD846:
Each batch was sequenced using Agilent's CREV4 (Clinical Research Exome) kit with a depth of 250x on Illumina platform. Raw reads are run through the Illumina DRAGEN Somatic Pipeline, which performs mapping, sorting and variant calling. The data has not been filtered.
HD867:
Each batch was sequenced using Agilent's CREV4 (Clinical Research Exome) kit with a depth of 250x on Aviti platform. Raw reads are run through the Illumina DRAGEN Somatic Pipeline, which performs mapping, sorting and variant calling. The data has not been filtered.
HD852, HD876, HD852-IVD, HD876-IVD:
Each batch was sequenced using Agilent's CREV4 (Clinical Research Exome) kit with a depth of 500x on Aviti platform. Raw reads are run through the Illumina DRAGEN Somatic Pipeline, which performs mapping, sorting and variant calling. The data has not been filtered.