Stability of siSTABLE siRNA in 100% Human Serum

The siSTABLE modification pattern dramatically extends the half-life of siRNA in the presence of 100% human serum. Unmodified siRNA degrades almost immediately while siSTABLE siRNA maintains integrity for up to 5 days.

Potent Silencing by siSTABLE Cyclophilin B siRNA

Western analysis of Cyclophilin B protein in HeLa cells was performed 96 and 120 hours after transfection with 100 nM of the indicated siRNAs: siSTABLE Non-Targeting siRNA #1 (D-001700-01, Lanes 1 and 4), siGENOME Cyclophilin B siRNA (D-001136-01, Lanes 2 and 5), and siSTABLE Cyclophilin B siRNA (D-001710-02, Lanes 3 and 6). Transfections were performed in 96-well plates with 5 x 103 cells/well and 0.2 μL DharmaFECT 1/well.

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