The Xenopus serves as a model organism for the study of embryonic development, developmental biology, cell biology, and oncogenesis. Xenopus resources include clones from the I.M.A.G.E. Consortium as well as Xenopus Gene Collection (XGC) clones for both Xenopus laevis and Xenopus tropicalis.
- Approximately 9000 clones representing almost 7800 unique gene IDs
- ORFs are derived directly from the XGC and placed in a Gateway Donor vector, omitting the stop codon, for easy transfer to an expression vector and 3ʹ end-tagging
- ORFs were end sequenced to confirm identity; detailed information on their development can be found here: http://www.xenbase.org/reagents/static/orfeome.jsp
XenopusGene Collection (XGC)
- A subset of the NIH Mammalian Gene Collection (MGC)
- Fully sequenced XGC clones for Xenopus laevis and Xenopus tropicalis
- Verified to contain a complete coding sequence (CDS)
- Individual clones, custom libraries, and entire collections available as glycerol stocks
- Xenopus Gene Collection (XGC)
I.M.A.G.E. Xenopus cDNA and EST clones
- Fully-sequenced clones were sequenced through the coding region of the gene by the I.M.A.G.E. consortium
- Partially-sequenced clones, also known as Expressed Sequence Tags (ESTs), have been 5’ or 3’ end-sequenced by the I.M.A.G.E. consortium and contain all or part of a specific gene
- I.M.A.G.E. Consortium website: http://image.hudsonalpha.org/
Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Scientific Support.
All clones and plates are provided as glycerol stocks. Individual clones are shipped at room temperature. Plates are shipped on dry ice. Both should be stored at -80°C.
- R. D. Morin et al., Sequencing and analysis of 10,967 full-length cDNA clones from Xenopus laevis and Xenopus tropicalis reveals post-tetraploidization transcriptome remodeling. Genome Res. 16(6), 796-803 (June 2006).
- MGC Project Team, The status, quality, and expansion of the NIH Full-length cDNA Project: The Mammalian Gene Collection (MGC). Genome Res. 14(10B), 2121-2127 (October 2004).
- R. L. Strausberg et al., The Mammalian Gene Collection. Science. 286(5439), 455-457 (15 October 1999).