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Yeast Genomic Tiling Collection

Designed to facilitate comprehensive overexpression screens, the Yeast Genomic Tiling Collection contains the yeast (Saccharomyces cerevisiae) genome in a 2 micron-based LEU2 vector.
dharmacon reagents

Created in Dr. Greg Prelich's laboratory at the Albert Einstein College of Medicine, the plasmid clones in the Yeast Genomic Tiling Collection represent a unique and virtually complete overlapping clone collection of the entire S. cervisiae genome. Plasmid inserts average ~10 kb with genes expressed untagged from their endogenous promoters. This clone collection constitutes a "minimal functional pathway" through the yeast genome, representing ~97% of the length of the yeast genome at the physical level and ~95% of the genome at the functional level.

Highlights

  • Allows efficient and comprehensive screens for overexpression phenotypes
  • Small number of plasmids (~1,500) allows systematic screens by direct transformation without any robots
  • All genes are untagged and expressed from their native promoters
  • Inserts contain all chromosomal elements, including protein-coding genes, non-protein-coding genes, intergenic regions, centromeres, and ARSs
  • Few examples of lethal plasmids are observed

Formats

Glycerol Stock Arrayed Library
  • Each clone has been mapped to the yeast genome
  • Create chromosome or pathway specific pools
  • Endless supply of DNA
Note

We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control.

Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. We are not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.

Glycerol stock arrayed format: plates are shipped on dry ice and should be stored at –80ºC.

Assay ready DNA format has been discontinued and is no longer available.