The Yeast Protein Interactome Collection allows for genome-wide in vivo screening of protein-protein interactions (PPI) in Saccharomyces cerevisiae.
First published in Science, Tarassov et al. used the Yeast Protein Interactome Collection to interrogate protein networks in Saccharomyces cerevisiae for systems-level behavior. This collection's high sensitivity is based on protein-fragment complementation and can detect as little as 25 to 100 complexes per cell. Using colony growth as an indication of PPI, this simple, but elegant selection strategy allowed the authors to systematically test over 15 million matings. The Yeast Protein Interactome Collection, with its published interactome, provides a basis to further explore individual networks or to test for genetic and environmental effects on a genome scale.
Detect structural and topological relationships between proteins in any subcellular location
Endogenous gene expression levels
High throughput assay allows screening of millions interactions
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