Mimix™ Non-Small Cell Lung Cancer Panel Negative Control, ctDNA Reference Standard
Non-Small Cell Lung Cancer Panel ctDNA Negative Controlは、ddPCRを用いて十分にテストされ、臨床的に関連する22のバリアントすべてについて陰性であることが確認されています。この細胞株を由来とするパネルは、ISO 9001:2015およびISO 13485:2016の下で製造されています。本パネルは、ネガティブコントロールとして、またはNSCLC ctDNA panel reference standard（HD844）のアレル頻度を希釈するために使用することができ、微小残存病変（minimal residual disease: MRD）のバリデーションをさらに有効にします。
Technical data
フォーマット： ctDNA
対象遺伝子 EGFR、KRAS、BRAF、MET、ERBB2、PIK3CA、NRAS
アレル頻度：0.00%
Buffer：Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Product information
フラグメント長：160 bp
ユニットサイズ：350 ng per vial
濃度：20 ng/µL
検証済み変異：
| DNA sequence change | Amino acid change | Expected allelic frequency (%) | |
|---|---|---|---|
| BRAF | c.1799T>A |
p.V600E |
0.00 |
| EGFR | c.2156G>C | p.G719A | 0.00 |
| EGFR | c.2303G>T | p.S768I | 0.00 |
| EGFR | c.2582T>A | p.L861Q | 0.00 |
| EGFR | c.2573T>G | p.L858R | 0.00 |
| EGFR | c.2235_2249del | p.E746_A750del | 0.00 |
| EGFR | c.2236_2250del | p.E746_A750del | 0.00 |
| EGFR | c.2240_2257del | p.L747_P753delisnS | 0.00 |
| EGFR | c.2369C>T | p.T790M | 0.00 |
| ERBB2 | N/A | Amplification | 2 copies |
| ERBB2 | c.2313_2324dup | p.Y772_A775dup | 0.00 |
| ERBB2 | c.2331_2339dup | p.G778_P780dup | 0.00 |
| KRAS | c.34G>T | p.G12C | 0.00 |
| KRAS | c.35G>T | p.G12V | 0.00 |
| KRAS | c.35G>A | p.G12D | 0.00 |
| MET | N/A | Amplification | 2 copies |
| MET | c.3082+1G>T | Unknown (Exon 14 skipping) | 0.00 |
| NRAS | c.181C>A | p.Q61K | 0.00 |
| NRAS | c.182A>T | p.Q61L | 0.00 |
| NRAS | c.182A>G | p.Q61R | 0.00 |
| PIK3CA | c.1624G>A | p.E542K | 0.00 |
| PIK3CA | c.3140A>G | p.H1047R | 0.00 |
General information
保管温度：4°C
有効期限：product shelf life informationをご参照ください。
使用目的：アッセイ開発、遺伝子解析用（研究用試薬）
Quality control
断片化サイズ：D1000 ScreenTape アッセイ
アレル頻度：Droplet Digital™ PCR
定量化：Qubit® dsDNA BRアッセイ（Invitrogen）
References
Kris MG, Camidge DR, Giaccone G, et al. Targeting HER2 aberrations as actionable drivers in lung cancers: phase II trial of the pan-HER tyrosine kinase inhibitor dacomitinib in patients with HER2-mutant or amplified tumors. Ann Oncol. 2015;26(7):1421-1427. doi:10.1093/annonc/mdv186
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Reference Standards
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At what temperature do you ship reference standards?
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How are Horizon cfDNA and ctDNA Reference Standards different from other circulating tumor DNA controls?
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How are Horizon cfDNA and ctDNA Reference Standards fragmented?
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How do you manufacture your Horizon Reference Standards?
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Is it possible to order custom versions of Horizon Reference Standard?
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Is there a wild-type control in the same background as NSCLC ctDNA Panel Reference Standard (HD844)?
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What are cfDNA Reference Standards?
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What is MRD?
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What is the difference between ctDNA and cfDNA?
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Which Droplet Digital PCR (ddPCR) system is used for variant detection at Horizon?
Related Products
Non-Small Cell Lung Cancer Panel ctDNA Reference Standardは、MRDアッセイのバリデーションにご使用いただけます。ISO 9001:2015およびISO 13485:2016の下で製造された、7遺伝子の22のバリアントを含む、十分に特徴付けられた細胞株を由来とする精度管理用試料です。