Expanded options for ordering custom guide RNAs from the CRISPR Design Tool
The CRISPR Design Tool is a popular tool for designing guide RNAs, and for ordering synthetic crRNA or lentiviral sgRNA for CRISPR-Cas9 gene editing at a specific target region. Keep reading to learn about new options for guide RNA design and ease of use in ordering!
Support for birds, reptiles, amphibians, mammals, fish, and more!
CRISPR-Cas9 has opened the door to precise gene editing in a wide variety of model organisms, but many predesigned product lines and online design tools are limited in the number of species they support. Without proper genome-wide alignment analysis against the organism of interest, there’s a risk of generating guide RNA designs that induce off-target cleavage. To address this, the updated version of the Dharmacon CRISPR Design Tool includes integrated genome tools for over 30 species! Want high-confidence lentiviral sgRNA for chimp? No problem! How about synthetic crRNA knockout reagents for pig? Sure thing! Check Table 1 for the list of available organisms. Still don’t see your species of interest? Please send a note to Scientific Support to let us know.
| Common name | Scientific name | Genome build |
| Human | Homo sapiens | hg38 |
| Mouse | Mus musculus | mm10 |
| Rat | Rattus norvegicus | rn6 |
| Zebrafish | Danio rerio | danRer10 |
| Western clawed frog | Xenopus tropicalis | xenTro7 |
| Fruit fly | Drosophila melanogaster | dm6 |
| Chinese hamster | Cricetulus griseus | criGri1 |
| Pig | Sus scrofa | susScr3 |
| Cow | Bos taurus | bosTau8 |
| Marmoset | Callithrix jacchus | calJac3 |
| Dog | Canis familiaris | canFam3 |
| Roundworm | Caenorhabditis elegans | ce10 |
| Sea Squirt | Ciona intestinalis | ci2 |
| Horse | Equus caballus | equCab2 |
| Cat | Felis catus | felCat8 |
| Fugu | Takifugu rubripes | fr3 |
| Chicken | gallus gallus | galGal4 |
| Stickleback | Gasterosteus aculeatus | gasAcu1 |
| Opossum | Monodelphis domestica | monDom5 |
| Ferret | Mustela putorius furo | musFur1 |
| Nile tilapia | Oreochromis niloticus | oreNil2 |
| Platypus | Ornithorhynchus anatinus | ornAna1 |
| Rabbit | Oryctolagus cuniculus | oryCun2 |
| Medaka | Oryzias latipes | oryLat2 |
| Sheep | Ovis aries | oviAri3 |
| Bonobo | Pan paniscus | panPan1 |
| Chimp | Pan troglodytes | panTro4 |
| Baboon | Papio Anubis | papAnu2 |
| Orangutan | Pongo pygmaeus abelii | ponAbe2 |
| Rhesus | Macaca mulatta | rheMac3 |
| Alligator | Alligator mississippiensis | allMis1 |
| Purple sea urchin | Strongylocentrotus purpuratus | strPur2 |
| Zebra finch | Taeniopygia guttata | taeGut2 |
Table 1: Species with integrated gene identifiers and genome-wide alignment capability in the CRISPR Design Tool
New lengths: The long and the short of guide RNAs
It is generally accepted that a 20 nt target sequence is the standard for CRISPR-Cas9 guide RNAs, but some experiments, especially those investigating mechanisms of specificity, may require a shorter or longer target region. Now when choosing the "Input my own guide RNA sequence" option on the CRISPR Design Tool, you may enter a target region from 17-23 nt with no change in price or delivery time of your custom guide RNA.
Multiple targets? Multiple genes? No problem.
To alleviate the time-consuming process of ordering multiple guide RNAs using your own target sequences, the CRISPR Design Tool input will now accept input of up to 200 targets at once! Simply enter or paste each target DNA sequence, one per line, in the input window. You also have the option of including a name for each design. If you’re ordering custom synthetic crRNA, you have the additional flexibility of having them delivered in a deep well plate (minimum of 20 wells), rather than tubes. Contact your sales representative or our customer service team for more details on this complementary service.