Mouse On-TARGETplus siRNA Library - Epigenetics

siRNA designed and modified for greater specificity

An arrayed collection of siRNA reagents for RNAi screening, targeting mouse enzymes involved in epigenetic regulation. ON-TARGETplus siRNA is guaranteed to silence, and is modified to reduce off-targets for fewer false positives.
dharmacon reagents

The ON-TARGETplus Mouse Epigenetics siRNA Library targets enzyme classes characterized to play a role in epigenetic mechanisms (see Reference 1). These inheritable factors that regulate genetic expression are of growing importance to scientific understanding of disease progression and trait inheritance. Modification of histones, such as methylation or acetylation, plays a key role in epigenetics as the extent to which DNA is wrapped around these protein complexes will alter the availability of genes in those DNA segments to be activated. The Epigenetics library targets genes which encode histone and DNA modification factors like methyltransferases, acetyltransferases, kinases and deacetylases, in addition to genes with a role in chromatin remodeling and histone ubiquitination.

The ON-TARGETplus siRNA designs and modifications reduce off-target effects while maintaining high silencing potency for high-confidence screening results.

Highlights
  • Patented dual-strand ON-TARGETplus modification pattern on all siRNAs to reduce off-targets
    • Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake
    • Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity
  • Available as SMARTpool siRNA reagents or a Set of 4 siRNAs in 96-well plates
  • Guaranteed target gene knockdown (seeSpecifications tab)
Gene Targets

For a complete list of target genes in this siRNA Library, please contact Scientific Support or your local Sales Representative.