DharmaFECT Duo Transfection Reagent

For high-confidence co-transfection

Optimized for co-transfection, DharmaFECT Duo transfection reagent provides highly effective delivery of siRNA, microRNA or crRNA reagents with plasmid. Useful for experiments utilizing reporter genes, RNAi rescue, or Edit-R Cas9 plasmid.

Updated shipping conditions: Horizon Discovery always strives to reduce its environmental impact. Sustainability goals factor in many decisions regarding our business. By eliminating Styrofoam from North America and Canada DharmaFECT packaging, Horizon will divert >110k cubic feet of long-decomposing material from landfills every year (that’s over 33 semi-trucks), and product efficacy remains guaranteed.

In side-by-side cotransfection experiments with traditional reagents developed for plasmid delivery, only DharmaFECT Duo Transfection Reagent resulted in efficient cell delivery of both siRNA (assayed by target gene knockdown) or microRNA reagents, along with plasmid (assayed by level of reporter expression).

DharmaFECT Duo is an excellent choice for experiments requiring cotransfection, such as Edit-R Gene Engineering, RNAi rescue or utilization of reporter systems.

Highlights

Effective delivery of two distinct molecules, DNA plasmid and small RNA, from a single formulation
Efficient transfection without compromising cell viability
Potent RNAi results together with plasmid expression

For transfection of plasmid DNA or shRNA, please select DharmaFECT kb DNA transfection reagent. For transfection of siRNA or microRNA only, please select DharmaFECT 1 Transfection Reagent.

Editing of PSMD7 gene in U2OS-(Ubi)EGFP cells using Edit-R Cas9 Nuclease protein NLS delivered by DharmaFECT transfection reagents

U2OS-(Ubi)EGFP cells were plated at 10,000 cells/well in 96-well plates and co-transfected using DharmaFECT transfection reagents with 25 nM Edit-R Cas9 Nuclease protein NLS and synthetic crRNA:tracrRNA at 50 or 100 nM targeting PSMD7. Cells were harvested 72 hours post-transfection and the relative frequency of gene editing was calculated based on a DNA mismatch detection assay with T7 Endonuclease I. UT = untreated sample, MW = FastRuler Low Range DNA Ladder (Thermo Scientific).

Editing of VEGFA gene in HEK293T cells using Edit-R Cas9 Nuclease mRNA delivered by DharmaFECT transfection reagents

HEK293T cells were plated at 20,000 cells/well in 96-well plates and co-transfected using DharmaFECT transfection reagents with 200 ng of Edit-R-Cas9 mRNA and synthetic crRNA:tracrRNA targeting VEGFA. Cells were harvested 72 hours post-transfection and the relative frequency of gene editing was calculated based on a DNA mismatch detection assay with T7 Endonuclease I. DF1 = DharmaFECT 1, Duo = DharmaFECT Duo, UT = untreated sample, MW = FastRuler Low Range DNA Ladder (Thermo Scientific).

DharmaFECT Duo is highly effective in co-transfection of plasmid with siRNA or microRNA

DharmaFECT Duo transfection reagent achieves reliable cotransfection results for a variety of experimental goals. The graph demonstrates specific modulation of Renilla luciferase (Rluc) gene:

Rluc expression is similar for Plasmid Alone and Plasmid+Control

Down-regulation of the Rluc as a result of miRNA mimic (plasmid + miRNA mimic)

Excellent knockdown of the Rluc by siRNA (Plasmid + siRNA)

The psiCHECK-2 vector (100 ng/well; Promega) with cloned microRNA recognition sites for miR-28, miR-95, miR-30d, and miR-105, respectively, were complexed alone or with RNAi reagents (10 nM) using DharmaFECT Duo (0.2 μL/well) in MCF-7 cells at 10,000 cells/well (96-well plate). The RNAi reagents were Dharmacon miRIDIAN Mimics (Negative Control, miR-28, miR-95, miR-30d, and miR-105, respectively) or siRNA (Renilla luciferase pool). Firefly and Renilla luciferase expression was assessed at 48 hours using the Dual-Glo Luciferase Assay System (Promega) and normalized to identically treated psiCHECK-2 empty vector.