The siGLO Red Transfection Indicator is a fluorescent oligonucleotide duplex that localizes to the nucleus, thus permitting unambiguous visual assessment of uptake into mammalian cells. It is ideal for use in optimization experiments to determine optimal siRNA, crRNA:tracrRNA, or microRNA reagent transfection conditions and for monitoring relative delivery efficiency.
Transfection Indicators are intended as a qualitative indicator of delivery and should not be used for quantitative estimation of experimental results (e.g. gene knockdown or knockout). They do not interact with RISC or Cas9 nuclease so have no gene silencing or DNA cleavage properties.
siGLO Red Transfection Indicator (Cy3) may show some punctate staining, but has a stronger signal at lower concentration than siGLO Green. It is suitable for transfection, electroporation or nucleofection.
- Cy3-labeled Transfection Indicator exhibits a strong signal at low concentration
- Absorbance/Emission Max is 547/563 nm
- A Cy3, Rhodamine or PE filter can be used
- Specially modified, fluorescent RNA duplex for simplified transfection assessment - without performing a functional assay
- Fluorescent signal localizes to the nucleus as an unmistakable signal of uptake
- Use as an indicator of transfection success in any experiment requiring delivery of synthetic RNA (siRNA, crRNA:tracrRNA, sgRNA, microRNA)
siGLO Red Transfection Indicator simplifies transfection optimization
Assessment of nuclear fluorescence permits easy optimization of conditions that result in efficient delivery into cells. siGLO transfection indicators are specially designed for nuclear localization for a bright, clear signal of transfection success. siGLO Red Transfection Indicator (50nM) was transfected into HeLa cells with DharmaFECT 1 (0.15 µg/100µL well). After 48 hours, cells were washed with 1x PBS and stained with Hoechst 3342 nuclear dye (blue).
siGLO Red Transfection Indicator does not interfere with functional siRNA
Cyclophilin B and Lamin A/C mRNA levels were quantified by RT-qPCR in HeLa cells 48 hours after transfection with DharmaFECT 1 (0.15 µg/100µL well) and 50nM of siGLO Cyclophilin B or Lamin A/C control, siGLO RISC-Free control, or siGLO Red Transfection Indicator.
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments
Concentrated buffer solution recommended for resuspension and long-term storage of any short, double-strand, or single-strand synthetic RNA molecule. Dilute with RNase-free water prior to use.
Molecular grade water for dilution of 5x siRNA Buffer or resuspension of RNA. RNase-free to prevent degradation of RNA reagents and oligonucleotides.