Make accurate and specific expression changes or alterations to any gene
Dharmacon™ reagents offer a complete suite of CRISPR reagents, including synthetic and lentiviral reagents, multiple nuclease delivery options, and custom design tools, to support every stage of your gene editing workflow.
These include sgRNA and Cas9/dCas9-effector solutions for CRISPR knock-out (CRISPRko), CRISPR knock-in (CRISPRki), CRISPR activation (CRISPRa), CRISPR interference (CRISPRi), and CRISPR derived base editing.
CRISPR knock-out (CRISPRko): Introduce double-strand breaks to generate knockouts for target validation, functional screens, and disease modeling.
CRISPR knock-in (CRISPRki): Insert DNA sequences via HDR for protein tagging, reporter integration, and mutation correction.
CRISPR activation (CRISPRa): Activate gene expression using dCas9-transcriptional activators for gene expression studies.
CRISPR interference (CRISPRi): Reversibly silence genes with dCas9 repressors for knockdown and epigenetic studies.
CRISPR derived base editing: Convert single DNA bases (C→T or A→G) without DSBs for precise point mutation correction and variant screening.
CRISPR knockout (CRISPRko)
CRISPR activation (CRISPRa)
CRISPR interference (CRISPRi)
CRISPR knock-in (CRISPRki)
Base editing
CRISPR controls
Essential controls for successful CRISPR-Cas9, CRISPR activation, and CRISPR interference experiments.
Transfection and transduction reagents
DharmaFECT™ transfection and transduction reagents as well as LentiBOOST™ transduction enhancer.
CRISPRko, CRISPRa, and CRISPRi libraries
Investigate entire gene families or biological pathways with our broad suite of custom and predefined CRISPR libraries.
