Note that the default design settings will generate CRISPR designs for gene knockout. If you wish to view and change the default design parameters, use Advanced Options ➜
PAM relative to target
Relative to Guide
Target sequence length
Cut relative to target 5' start
To generate guide options adjust design parameters by launching Advanced Options below.
For help in setting design parameters please
reach out to our Scientific Support team.
In some cases, no design results are returned using the default parameters. Below are potential causes and suggestions for modifying the default settings to obtain designs:
In some cases, no crRNA design results are returned using the default parameters. Below are examples of why the default settings may return no crRNA designs, and suggestions for obtaining designs:
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