Product Insert - Option A1 custom siRNA synthesis
Option A1 custom siRNA synthesis product insert, containing contents, protocol, shipping and storage conditions, handling precautions, etc.
Large scale and specialized oligo production
Accelerate your research with scalable, specialized, and high-quality oligonucleotide manufacturing
From early-stage discovery to preclinical validation, your research demands oligonucleotides that meet the highest standards of quality, purity, and consistency. Dharmacon Reagents large scale and specialized oligo services deliver custom oligonucleotides engineered for both in vitro research and in vivo preclinical applications - giving academic institutions and industry partners the reliable materials needed to advance breakthrough science.
Whether you're working on novel research, validating targets, or scaling up for preclinical studies, we provide cost-effective, timely manufacturing solutions that grow with your project. Our expertise in large-scale oligo synthesis ensures you receive the precise sequences you need, when you need them, without compromising quality or your timeline.
Why choose Dharmacon Reagents large scale oligo services?
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ISO 9001-certified
- Our manufacturing facility meets rigorous international quality standards, backed by a highly trained and dedicated custom oligonucleotide synthesis team committed to delivering exceptional results.
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Scalable manufacturing for your needs
- Preclinical-scale synthesis - from 50 mg to multiple grams.
- Flexible chemistry options - proprietary 2'-ACE or 2'-TBDMS synthesis methods tailored to your requirements.
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Extensive customization capabilities
- Chemical modifications - modified nucleobases, phosphorothioates, linkers, spacers, and fluorescent dyes. A full list of modifications available on our web ordering tools can be found at our Bases and Modifications page.
- OEM and contract production - customized oligonucleotide reagents designed to meet your exact specifications.
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Custom support available
- Request modifications or customized purification and processing beyond the standard catalog or get design guidance from our team. Contact our scientific support team.
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Trusted partner for innovation
- Supporting academic and industry researchers with fast turnaround times, rigorous QC processes, and the flexibility to adapt to evolving project needs.
- All oligos made by the Dharmacon Reagents team are examined internally by mass spectrometry. Additionally, ultra-HPLC analytical data and mass spec readouts are available on request as an additional service.
- Backed by 30+ years of oligo synthesis experience and trusted by researchers worldwide.
Supporting data
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Dharmacon large-scale custom synthesis capabilities
To demonstrate the quality, consistency, and scalability of our oligonucleotide manufacturing services, we're pleased to share the following data:
Purified 42-mer crRNA
B
C
Purity analysis and characterization of a 42-mer crRNA oligonucleotide. The 42-mer RNA was synthesized using 2’-O-TBDMS chemistry at 120 µmol scale. After cleavage and deprotection, the RNA oligonucleotide was purified by anion-exchange chromatography. Pooled fractions were desalted by ultra-filtration followed by freeze drying to yield lyophilized RNA. (A) Analytical ion-pairing reverse phase UPLC trace showing 92% full-length product (FLP); (B) Analytical anion-exchange HPLC trace showing 89% full-length product (FLP); (C) Mass was analyzed with the LXQ Mass Spectrometer, confirming the correct mass.
Purified 30-mer DNA
A
B
Analysis of a 30-mer DNA oligonucleotide with final delivery yield of 1 g. (A) Analytical trace from ion pairing reverse phase UPLC showing 97% full length material. (B) Mass was analyzed by ESI-MS, results illustrate the correct mass.
These data highlight our commitment to delivering the high-purity, custom-tailored oligonucleotides our partners require to drive their most ambitious research initiatives forward. By partnering with us, you can be confident your projects will have access to the scalable, reliable materials needed to accelerate discovery and validation.
Ready to take your research to the next level? Let's discuss how our large-scale oligo synthesis capabilities can support your goals.
Additional resources
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Custom synthesis brochure
Dharmacon custom RNA synthesis enables additional experimental abilities and scientific discoveries through unique chemistry.
Read more
Technical documents
Product Insert - Option A4 custom siRNA synthesis
Option A4 custom siRNA synthesis product insert, containing contents, protocol, shipping and storage conditions, handling precautions, etc.
Product Insert - Option in vivo and in vivo HPLC custom siRNA synthesis
Dharmacon option in vivo: The siRNA is shipped as a lyophilized pellet in the 2'- deprotected and duplexed form.
Protocol - Deprotection of 2'-ACE protected RNA
How to deprotect 2'-ACE protected RNA
Protocol - Ethanol precipitation of RNA oligonucleotides
This protocol is for Ethanol Precipitation of RNA Oligonucleotides.
Protocol - Reverse phase cartridge desalting of RNA oligonucleotides
Protocol for desalting RNA oligos using reverse phase cartridges, including protocol for cartridge equilibration, washing, and eluting from cartridge.
Protocol - TCEP reaction for thiol-modified siRNA/RNA oligonucleotides
Protocol to deprotect (reduce) oxidized thiol-modified siRNA and RNA oligonucleotides.
Recommended reading
Publication list highlighting Dharmacon reagent development and collaborations across CRISPR-Cas9, RNAi, and oligo synthesis.
Safety data sheets
SDS (US) RNA oligonucleotides
North American standard safety data sheet for RNA oligonucleotides.
SDS (US) deprotection buffer
North American standard safety data sheet for deprotection buffer.
SDS (EU) RNA oligonucleotides
European Union standard safety data sheet for RNA oligonucleotides.
SDS (EU) deprotection buffer
European Union standard safety data sheet for deprotection buffer.