CRISPR Screening Libraries
Achieve high specificity and functionality in CRISPR screens.
Explore selected gene families or pathways, druggable targets, and the whole genome with our comprehensive suite of custom and pre-defined CRISPR screening libraries - designed for CRISPR knock-out (CRISPRko), activation (CRISPRa), and interference (CRISPRi) in both arrayed and pooled screening.
Our algorithm-optimized Edit-R guide RNA designs for CRISPRko deliver precise and consistent knockout and reproducibility across both set-of-3 individual or pooled synthetic sgRNA arrayed libraries and 4 or 8 sgRNA per gene pooled lentiviral library formats. Combined with curated offerings of genome-wide pre-designed CRISPRa and CRISPRi sgRNAs in both synthetic arrayed and pooled lentiviral formats you have the flexibility to scale from targeted arrayed studies to genome-wide screens.
Explore screening platforms
CRISPR knock-out
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Edit-R synthetic sgRNA libraries for CRISPRko
Arrayed CRISPR libraries in individual and pooled sgRNA per well formats. Predesigned gene family to whole-genome for human and mouse. -
Edit-R lentiviral sgRNA pooled libraries for CRISPRko
High-titer pooled sgRNA screening libraries. Predesigned gene family to whole-genome collections available for human and mouse. -
Edit-R Human All-in-one lentiviral sgRNA pooled libraries for CRISPRko
All-in-one vectors expressing Cas9 nuclease plus sgRNA in the same vector for simplified delivery and selection in pooled screening applications
CRISPR interference
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CRISPRmod lentiviral sgRNA pooled libraries for CRISPRi
High-titer pooled sgRNA screening libraries for predefined gene sets or the whole genome. -
CRISPRmod All-in-one lentiviral sgRNA pooled libraries for CRISPRi
All-in-one vectors expressing dCas9-SALL1-SDS3 plus sgRNA in pools for simplicity in pooled screening. -
CRISPRmod synthetic sgRNA libraries for CRISPRi
Order Cherry-pick arrayed synthetic sgRNA libraries with CRISPRi predesigns in pool of 3 or individual sgRNA per well format here.
CRISPR activation
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CRISPRmod synthetic sgRNA libraries for CRISPRa
High-throughput CRISPR activation. Choose predesigned arrayed gene family to whole- genome collections in individual and pooled sgRNA per well formats. -
CRISPRmod lentiviral sgRNA pooled libraries for CRISPRa
High-titer pooled sgRNA screening libraries for predefined gene sets or the whole genome. -
CRISPRmod All-in-one lentiviral sgRNA pooled libraries for CRISPRa
All-in-one vectors expressing dCas-VPR plus sgRNA in the same vector for simplified delivery and selection in pooled screening applications
Custom screening libraries & accessories
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Cherry-pick arrayed synthetic guide RNA libraries
Order customized synthetic guide RNA plates for CRISPRko, CRISPRa, or CRISPRi. Customize plating with controls and more. -
Custom pooled lentiviral sgRNA libraries
Order custom pools in sgRNA alone or All-in-one formats for CRISPRko, CRISPRi, or CRISPRa -
Transfection and transduction reagents
DharmaFECT™ transfection and transduction reagents as well as LentiBOOST™ transduction enhancer. -
tracrRNA
Chemically synthesized, HPLC-purified tracrRNA that pairs with crRNA to recruit Cas9 nuclease. -
Custom sgRNA plates
Order arrays of tubes or plates of customized sgRNA sequences through our custom oligo solutions.
Why choose Dharmacon reagents CRISPR libraries
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Investigate entire gene families, biological pathways, drug targets, or the whole genome
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Algorithm-optimized guide RNA designs for CRISPR knock-out in sets of individual guides or pools of guides for both arrayed and pooled screening applications
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Pre-designed CRISPRa and CRISPRi guide RNAs for both arrayed and pooled screening applications
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All-in-one nuclease and sgRNA lentiviral pooled library options for simplicity
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Custom or pre-defined offering unique flexibility
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Matching pre-defined library compositions between CRISPR synthetic arrayed or pooled sgRNA libraries and siRNA arrayed screening libraries for unmatched orthogonal flexibility between screening methods and gene perturbation technologies
Choose the right platform
Choosing the right CRISPR-Cas9, CRISPR activation, or CRISPR interference screening platform starts with understanding your assay. Use the table below to compare lentiviral pooled sgRNA libraries and synthetic sgRNA/crRNA libraries across product formats, delivery, assay type, and data analysis requirements.
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Lentiviral pooled sgRNA libraries |
Synthetic sgRNA/crRNA libraries |
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Format |
Lentiviral particles in a small number of tubes |
Synthetic sgRNA/crRNA arrayed in multi-well plates |
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Delivery to cells |
Lentiviral transduction |
Standard transfection reagents or electroporation |
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Assay types supported |
Phenotypes that result in changes in sgRNA abundance in the cell population and can be assessed by NGS can be investigated. Growth phenotypes (proliferation or survival) Selectable by cell sorting (fluorescence or cell surface marker expression) |
A wide range of cellular phenotypes (high content analysis, reporter or enzymatic assays, etc.) can be investigated due to one-gene-one-well layout. Editing efficiency in the population must be sufficient to allow for observation of the specific phenotype. |
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Screening 'hands-on' time |
Relatively low; can be carried out in a single culture dish |
Scales up with the number of genes |
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Data analysis requirements |
Next-gen sequencing required for identification of sgRNA(s) and its abundance in the cell population |
Phenotype is analyzed directly on a one-gene-per-well basis |
Featured resources
Carousel items
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Arrayed or pooled library? Make the right first move in your CRISPR screen.
Read about the fundamental principles that drive the decision – to pool or not to pool? -
Why using orthogonal loss-of function approaches can strengthen your genetic research.
Learn about how using complementary gene perturbation technologies in your screen can increase your confidence in results. -
Using CRISPR Single-Cell Screening to Reveal Gene Function at High Resolution
Learn how researchers are combining pooled screening methods with single-cell technologies to unlock a new layer of sensitivity.
