CRISPR knockout ready cells
- Generated using Horizon's own Edit-R lentiviral vectors with a Blasticidin resistance selection marker.
- QC verified and validated to ensure stable expression and functionality of Cas9 endonuclease.
- Cells are provided in a pooled format of >1 million cells per vial.
Ideal for use with other Horizon products
For gene editing experiments and loss-of -unction studies:
For screening projects
Limited use agreement
All cell lines engineered by Horizon Discovery are purchased under the terms of our Limited Use Label License.
Cell culture medium and cell line maintenance tips
|Cell line background||Cat #||Basal medium||Supplements||Subculturing tips|
|A549||HD Cas9-001 |
|RPMI 1640||10% FBS |
|HCT-116||HD Cas9-002 |
|RPMI1640||10% FBS |
|Jurkat||HD Cas9-004||RPMI1640||10% FBS |
|K-562||HD Cas9-005 |
|IMDM||10% FBS |
|RKO||HD Cas9-007||RPMI1640||10% FBS |
|NIH/3T3||HD Cas9-010||DMEM, high glucose, GlutaMAXTM||10% FBS |
|HAP1||HD Cas9-011 |
|IMDM||10% FBS |
|U2OS||HD Cas9-012 |
|DMEM, high glucose||10% FBS |
1% Sodium Pyruvate
|MDA-MB-231||HD Cas9-014 |
|DMEM/F-12||10% FBS |
Efficient indel formation using Edit-R™ synthetic sgRNA in Cas9 expressing stable cell lines
Adherent Cas9 Stable Cell Lines were plated at 10,000 cells/well and reverse transfected using DharmaFECT1 or DharmaFECT4 Transfection Reagent with synthetic sgRNA (25 nM) targeting PPIB (Cat # U-009000-01-XX). Cells were harvested 72 hours post-transfection and the relative efficiency of indel formation was assessed using trace decomposition (TIDE) compared to a non-targeting control (NTC; Cat # U-009501-01-XX).
Suspension Cas9 Stable Cell Lines were electroporated using 100,000 cells/well, and Lonza buffer SE or SF, respectively, with synthetic sgRNA (5 µM) targeting PPIB (Cat # U-009000-01-XX). Cells were harvested 72 hours post-transfection and the relative efficiency of indel formation was assessed using trace decomposition (TIDE) compared ion to a non-targeting control (NTC; Cat # U-009501-01-XX).
|Shipping Condition||Dry Ice|
|Storage Conditions||Liquid N2|
|Shelf Life||60 Months|
|Stability at Recommended Storage Conditions||At least 24 months|