Cas9 Stable Cell Lines

Simplify gene editing experiments with stably expressing Cas9 cell lines

Choose from a range of popular cell line backgrounds, then simply deliver your CRISPR guide RNA for straightforward gene knockout.

Generated using Horizon's own Edit-R lentiviral vectors with a Blasticidin resistance selection marker.
QC verified and validated to ensure stable expression and functionality of Cas9 endonuclease.
Cells are provided in a pooled format of >1 million cells per vial.

For gene editing experiments and loss-of-function studies:

For screening projects

All cell lines engineered by Horizon Discovery are purchased under the terms of our Limited Use Label License.

Cell culture medium and cell line maintenance tips

Cell line background	Cat #	Basal medium	Supplements	Subculturing tips
A549	HD Cas9-001	RPMI 1640	10% FBS 1% Pen/Strep	Recommended split ratio - 1:3 to 1:8 Maintain cultures between 6 x 10³
HCT-116	HD Cas9-002 HD dCas9-VPR-002	RPMI1640	10% FBS 1% Pen/Strep	Recommended split ratio - 1:3 to 1:8 Split cultures at 75-90% confluency
Jurkat	HD Cas9-004	RPMI1640	10% FBS 1% Pen/Strep	Maintain cultures between 1 x 10⁵ and 1 x 10⁶ cells/mL
K-562	HD Cas9-005 HD dCas9-VPR-005	IMDM	10% FBS 1% Pen/Strep	Maintain cultures between 1 x 10⁵ and 1 x 10⁶ cells/mL
RKO	HD Cas9-007	RPMI1640	10% FBS 1% Pen/Strep	Recommended split ratio - 1:3 to 1:12 Split cultures at 75-90% confluency
NIH/3T3	HD Cas9-010	DMEM, high glucose, GlutaMAX^TM	10% FBS 1% Pen/Strep	Seed 3-5 x 10³ cells/cm² Split cultures at 80% confluence or less Do not allow the cultures to become confluent
HAP1	HD Cas9-011 HD dCas9-VPR-011	IMDM	10% FBS 1% Pen/Strep	Recommended split ratio - 1:10 - 1:15 Do not allow cells to be kept at high density, maximum density: 75% confluency
U2OS	HD Cas9-012 HD dCas9-VPR-012	DMEM, high glucose	10% FBS 1% Sodium Pyruvate 1% Pen/Strep	Recommended split ratio - 1:3 to 1:6 Split at 75-90% confluency