Cas9 Stable Cell Lines
Simplify gene editing experiments with stably expressing Cas9 cell lines
Choose from a range of popular cell line backgrounds, then simply deliver your CRISPR guide RNA for straightforward gene knockout.
Cas9 stable cell lines are QC verified and validated to ensure stable expression and functionality of Cas9 endonuclease.
- Generated using Horizon's own Edit-R Lentiviral vectors with a Blasticidin resistance selection marker.
- Cells are provided in a pooled format of >1 million cells per vial.
Ideal for use with other Horizon products:
For gene editing experiments and loss of function studies:
Cell culture medium and cell line maintenance tips
Cell line background | Cat # | Basal medium | Supplements | Subculturing tips |
---|---|---|---|---|
A549 | HD Cas9-001
HD dCas9-VPR-001 |
RPMI 1640 | 10% FBS
1% Pen/Strep |
|
HCT-116 | HD Cas9-002
HD dCas9-VPR-002 |
RPMI1640 | 10% FBS
1% Pen/Strep |
|
Jurkat | HD Cas9-004 | RPMI1640 | 10% FBS
1% Pen/Strep |
|
K-562 | HD Cas9-005
HD dCas9-VPR-005 |
IMDM | 10% FBS
1% Pen/Strep |
|
RKO | HD Cas9-007 | RPMI1640 | 10% FBS
1% Pen/Strep |
|
NIH/3T3 | HD Cas9-010 | DMEM, high glucose, GlutaMAXTM | 10% FBS
1% Pen/Strep |
|
HAP1 | HD Cas9-011
HD dCas9-VPR-011 |
IMDM | 10% FBS
1% Pen/Strep |
|
U2OS | HD Cas9-012
HD dCas9-VPR-012 |
DMEM, high glucose | 10% FBS
1% Sodium Pyruvate 1% Pen/Strep |
|
MDA-MB-231 | HD Cas9-014
HD dCas9-VPR-014 |
DMEM/F-12 | 10% FBS
1% Pen/Strep |
|
Efficient indel formation using Edit-R™ synthetic sgRNA in Cas9 expressing stable cell lines
Adherent Cas9 Stable Cell Lines were plated at 10,000 cells/well and reverse transfected using DharmaFECT1 or DharmaFECT4 Transfection Reagent with synthetic sgRNA (25 nM) targeting PPIB (Cat # U-009000-01-XX). Cells were harvested 72 hours post-transfection and the relative efficiency of indel formation was assessed using trace decomposition (TIDE) compared to a non-targeting control (NTC; Cat # U-009501-01-XX).
Suspension Cas9 Stable Cell Lines were electroporated using 100,000 cells/well, and Lonza buffer SE or SF, respectively, with synthetic sgRNA (5 µM) targeting PPIB (Cat # U-009000-01-XX). Cells were harvested 72 hours post-transfection and the relative efficiency of indel formation was assessed using trace decomposition (TIDE) compared ion to a non-targeting control (NTC; Cat # U-009501-01-XX).
Shipping Condition | Dry Ice | |
Storage Conditions | Liquid N2 | |
Shelf Life | 60 Months | |
Stability at Recommended Storage Conditions | At least 24 months | |
Hazardous | No |