Cas9 Stable Cell Lines

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Cas9 stable cell lines are QC verified and validated to ensure stable expression and functionality of Cas9 endonuclease.

Generated using Horizon's own Edit-R Lentiviral vectors with a Blasticidin resistance selection marker.
Cells are provided in a pooled format of >1 million cells per vial.

Ideal for use with other Horizon products:

For gene editing experiments and loss of function studies:

Cell culture medium and cell line maintenance tips

Cell line background	Cat #	Basal medium	Supplements	Subculturing tips
A549	HD Cas9-001 HD dCas9-VPR-001	RPMI 1640	10% FBS 1% Pen/Strep	Recommended split ratio - 1:3 to 1:8 Maintain cultures between 6 x 10³
HCT-116	HD Cas9-002 HD dCas9-VPR-002	RPMI1640	10% FBS 1% Pen/Strep	Recommended split ratio - 1:3 to 1:8 Split cultures at 75-90% confluency
Jurkat	HD Cas9-004	RPMI1640	10% FBS 1% Pen/Strep	Maintain cultures between 1 x 10⁵ and 1 x 10⁶ cells/mL
K-562	HD Cas9-005 HD dCas9-VPR-005	IMDM	10% FBS 1% Pen/Strep	Maintain cultures between 1 x 10⁵ and 1 x 10⁶ cells/mL
RKO	HD Cas9-007	RPMI1640	10% FBS 1% Pen/Strep	Recommended split ratio - 1:3 to 1:12 Split cultures at 75-90% confluency
NIH/3T3	HD Cas9-010	DMEM, high glucose, GlutaMAX^TM	10% FBS 1% Pen/Strep	Seed 3-5 x 10³ cells/cm² Split cultures at 80% confluence or less Do not allow the cultures to become confluent
HAP1	HD Cas9-011 HD dCas9-VPR-011	IMDM	10% FBS 1% Pen/Strep	Recommended split ratio - 1:10 - 1:15 Do not allow cells to be kept at high density, maximum density: 75% confluency
U2OS	HD Cas9-012 HD dCas9-VPR-012	DMEM, high glucose	10% FBS 1% Sodium Pyruvate 1% Pen/Strep	Recommended split ratio - 1:3 to 1:6 Split at 75-90% confluency
MDA-MB-231	HD Cas9-014 HD dCas9-VPR-014	DMEM/F-12	10% FBS 1% Pen/Strep	Recommended split ratio - 1:2 to 1:4 Split cultures at 75-90% confluency