This tool is for designing and ordering the DNA components required to build a custom donor plasmid for insertion of EGFP, mKate2, or a custom sequence to any genomic site.

To design a guide RNA to cut near the genomic alteration site of interest, first use the CRISPR Design Tool.

If you already have a guide RNA that is near the desired insertion site, enter the gene information and 20 nt guide RNA target sequence below.

Need some additional guidance? Visit the HDR Donor Designer Workflow page to learn more.

After target