CRISPRmod CRISPRi synthetic sgRNA positive controls
Validated CRISPRi synthetic pools or individual sgRNA for optimization of transcriptional repression experiments
Used to verify optimal experimental conditions and ensure dCas9-SALL1-SDS3 expression for efficient gene repression
CRISPRi synthetic sgRNA positive controls will selectively repress a well characterized human gene (either PPIB1 or SEL1L). Positive controls are recommended for optimization and ongoing monitoring of delivery conditions and CRISPRi dCas9-SALL1-SDS3 expression prior to experiments with target gene-specific guide RNAs.
Highlights
- Select PPIB1 or SEL1L as your positive control gene
- Choose individual or pooled CRISPRi sgRNA controls to match your experimental CRISPRi sgRNA reagent format
- Ideal for optimization of experimental conditions and confirming successful gene repression
In addition to positive controls, it is recommended to use a CRISPRi synthetic sgRNA non-targeting control to determine baseline levels of gene expression.
