Guide RNA designer

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Revvity is committed to biosecurity and the responsible synthesis of oligonucleotides. For more information on how the Dharmacon Reagents are screened and how we adhere to the US Framework for Nucleic Acid Synthesis, please visit our Synthetic Oligonucleotide Sequence Screening page.

Guide RNA Designer

For use with S.pyogenes Cas9 (synthetic sgRNA, crRNA, or lentiviral sgRNA)

Enter a 17-23 nt target sequence. Repeat region will be appended automatically

For use with any Cas9 species

crRNA: Enter 35-45 nt consisting of target sequence and repeat region

Synthetic sgRNA: Enter 97-103 nt consisting of target sequence, repeat region, linker, and tracrRNA

Pin-point™ system: For use with ABE or CBE Pin-point base editing mRNAs (synthetic sgRNA)

Enter a 20 nt target sequence. Repeat region will be appended automatically

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