The miRIDIAN microRNA Hairpin Inhibitor Positive Control is ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for loss-of-function microRNA studies.
Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize inhibition experimental conditions by assessing the effect of inhibition on an endogenously expressed microRNA, we offer a validated Hairpin Inhibitor Positive Control.
- Targets endogenous miR-16 in human, mouse, and rat cells resulting in reduced miR-16 levels (seeFigure 1, Supporting Data)
- Control designs and modifications are identical to experimental miRIDIAN microRNA Hairpin Inhibitors
- Optimize microRNA inhibition assays by targeting a microRNA in a cell line that expresses miR-16
- Specific inhibition of miR-16 can be directly assessed by RT-qPCR and/or Northern blotting
2 nM of miRIDIAN miR-16 Hairpin Inhibitor is sufficient to bind or sequester a majority of endogenous miR-16
Figure 1. | Endogenous levels of miR-16 were assayed 48 hours after transfection of either miRIDIAN miR-16 Hairpin Inhibitor, miR-21 Hairpin Inhibitor or Hairpin Inhibitor Negative Control #1. HeLa cells were transfected using DharmaFECT 1.
|Storage Conditions||-20 C|
|Stability at Recommended Storage Conditions||At least 12 months|
Choose from two universal miRIDIAN Hairpin Inhibitor Negative Controls for experiments with miRIDIAN microRNA Hairpin Inhibitors. Negative control sequences based on <em>C. elegans</em> microRNAs have minimal sequence identity in human, mouse, and rat.
Choose from two universal miRIDIAN Hairpin Inhibitor Negative Controls for experiments with miRIDIAN microRNA Hairpin Inhibitors. Negative control sequences based on <em>C. elegans </em>microRNAs have minimal sequence identity in human, mouse, and rat.
The miRIDIAN microRNA Hairpin Inhibitor Transfection Control is a Cy3-labeled microRNA Hairpin Inhibitor based on the C. elegans miRNA cel-miR-67 (miRIDIAN Hairpin Inhibitor Negative Control #1) for monitoring delivery into human, mouse, and rat cells.