GFP Duplex I
In functional testing, the GFP Duplex I control siRNA has demonstrated high efficiency silencing of the green fluorescent protein reporter gene (GFP/eGFP) at both the mRNA and protein levels.
Because the GFP reporter sequence has been altered by various sources to improve its performance, we recommend aligning the siRNA target sequence to your reporter to ensure a sequence match. This siRNA control does not target TurboGFP.
Highlights
- Target sequence: 5’-
GCA AGC TGA CCC TGA AGT TC–3’ - Appropriate for use in any mammalian cell type
- N. J. Caplen, S. Parrish, F. Imani, A. Fire, R. A. Morgan, Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc Natl Acad Sci U S A. 98, 9742-9747 (14 August 2001).
Application notes
Safety data sheets
Related Products
Concentrated buffer solution recommended for resuspension and long-term storage of any short, double-strand, or single-strand synthetic RNA molecule. Dilute with RNase-free water prior to use.
Catalog ID:B-002000-UB-100
Unit Size:100 mL
$99.00
Molecular grade water for dilution of 5x siRNA Buffer or resuspension of RNA. RNase-free to prevent degradation of RNA reagents and oligonucleotides.
Catalog ID:B-003000-WB-100
Unit Size:100 mL
$32.00
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments
Catalog ID:T-2001-01
Unit Size:0.2 mL