When co-transfected with the reporter plasmid pGL3 (Promega) into mammalian cells, the Luciferase GL3 Duplex control siRNA effectively reduces GL3 expression.
Because the luciferase reporter sequence is often altered to improve its performance, we recommend aligning the siRNA target sequence to your reporter to ensure a sequence match.
- Target Sequence:
5'- CTT ACG CTG AGT ACT TCG A -3'
- Appropriate for use in any mammalian cell type
- S. M. Elbashir et al., Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411(6836), 494-498 (24 May 2001).
Concentrated buffer solution recommended for resuspension and long-term storage of any short, double-strand, or single-strand synthetic RNA molecule. Dilute with RNase-free water prior to use.
Molecular grade water for dilution of 5x siRNA Buffer or resuspension of RNA. RNase-free to prevent degradation of RNA reagents and oligonucleotides.
Optimized for co-transfection, DharmaFECT Duo transfection reagent provides highly effective delivery of siRNA, microRNA or crRNA reagents with plasmid
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments