ORFeome Collaboration Clones
ORFeome Collaboration Clones are fully-sequenced human open reading frames (ORFs) subcloned into Gateway-entry vectors and are available with or without native stop codons.
Please note that we are unable to provide additional information beyond what is listed on this page. The original source projects (e.g., MGC, ORFeome, IMAGE Consortium, DKFZ, Kazusa) no longer maintain active databases, and these legacy biological collections have limited validation. As a result, they are offered "as is" without guarantee.
To help ensure you receive the correct clone, we recommend isolating and sequencing multiple colonies (3-4) and conducting your own verification to confirm suitability for your research. Thank you for your understanding.
Attention
After searching for a gene, please review the insert sequence found under the sequence tab before completing your purchase.
ORFeome Collaboration Clones
1Start Here
The ORFeome Collaboration (OC) is a group of academic and commercial laboratories that was formed to develop an unrestricted source of sequence-validated human open reading frame (ORF) clones. This collaboration of commercial and academic researchers has created a collection of fully-sequenced ORFs that will represent all curated genes in the human genome. These clones are verified to have a full-length ORF and are easily transferable into any Gateway-adapted expression vector. Additional clones for this expanding collection are offered as they become available.
Dharmacon is a major distributor for the OC, providing individual clones, gene family libraries and whole-genome collections to academic and commercial customers.
Using the Mammalian Gene Collection (MGC) cDNA clones as starting material, the OC has been able to create over 10,000 full-length human ORF clones, making significant progress toward the goal of providing at least one ORF for each of the ~18,500 defined human genes.
Highlights:
- Fully-sequenced human open reading frames
- Gateway-adapted ORFs allow for efficient transfer into a variety of compatible expression vectors
- Inclusion of native stop codons allows for expression of native proteins
- Removal of stop codons allows for expression of C-terminal fusion proteins
Note
We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control.
Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. We are not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.
Individual Clones
Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. We will ship your clone at room temperature via express delivery. Store the stock clone at -80°C.
Bulk Orders
Orders of 50 clones or greater and stock plates will be provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.
Entire Collection
Orders for the entire promoter collection are provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for estimated shipping time on orders for the entire collection.