Xenopus

Fully-sequenced Xenopus Gene Collection (XGC) and IMAGE cDNA clones contain full coding sequences of expressed genes from Xenopus laevis and Xenopus tropicalis.
Dharmacon

dio3 ( Western Clawed Frog )

Entrez Gene 100038242 entrezgene 100038242
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deiodinase, iodothyronine, type 3

Alias

5DIII | DI-3 | dioiii | txdi3

The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. As in other verterbrates, studies in xenopus show that this enzyme is involved in early embryonic development. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016].

The Xenopus serves as a model organism for the study of embryonic development, developmental biology, cell biology, and oncogenesis. Xenopus resources include clones from the I.M.A.G.E. Consortium as well as Xenopus Gene Collection (XGC) clones for both Xenopus laevis and Xenopus tropicalis.

Full Xenopus genome collections of cDNA and ORF clones are also available.

Highlights

Xenopus ORFeome
  • Approximately 9000 clones representing almost 7800 unique gene IDs
  • ORFs are derived directly from the XGC and placed in a Gateway Donor vector, omitting the stop codon, for easy transfer to an expression vector and 3ʹ end-tagging
  • ORFs were end sequenced to confirm identity; detailed information on their development can be found here: http://www.xenbase.org/reagents/static/orfeome.jsp
Xenopus Gene Collection (XGC)
I.M.A.G.E. Xenopus cDNA and EST clones
  • Fully-sequenced clones were sequenced through the coding region of the gene by the I.M.A.G.E. consortium
  • Partially-sequenced clones, also known as Expressed Sequence Tags (ESTs), have been 5’ or 3’ end-sequenced by the I.M.A.G.E. consortium and contain all or part of a specific gene
  • I.M.A.G.E. Consortium website: http://image.hudsonalpha.org/

Disclaimer

Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Scientific Support.

All clones and plates are provided as glycerol stocks. Individual clones are shipped at room temperature. Plates are shipped on dry ice. Both should be stored at -80°C.